Prof. Dr. Jack Rohrer

Persönliches Profil

Leitungsfunktion

Leitung Fachgruppe Zellphysiologie und Zell-Engineering

Tätigkeit an der ZHAW

Leiter der Fachgruppe Zellphysiologie und Zell-Engineering, Spezialgebiete: Glykobiologie, rekombinante Proteinexpression, Entwicklung zellbasierender Assays, induzierte pluripotente Stammzellen, subzelluläres Drug Delivery, lysosomale Biogenese und generell molekulare Mechanismen des intrazellulären Proteintransportes.

www.zhaw.ch/de/lsfm/institute-zentren/icbt/zellbiologie-und-tissue-engineering/zellphysiologie-und-zell-engineering/

Arbeits- und Forschungsschwerpunkte, Spezialkenntnisse

Spezifische technische Kompetenzen beinhalten Mikroskopie (einschliesslich konfokaler Mikroskopie und life imaging) sowie eine Vielzahl an Methoden aus den Gebieten Molekularbiologie, Biochemie, Zellbiologie und Immunologie. Forschungsschwerpunkte sind induzierte pluripotente Stammzellen, Herstellung neuer Antikörper gegen komplexe Proteine, der Aufbau von Zell-basierenden Assays, und subzelluläres Drug Delivery.

Aus- und Fortbildung

Studium Biologie II an der Universität Basel (1984-1988).
Dissertation am Biozentrum in der Abteilung Biochemie der Universität Basel (1988-1992).
PostDoc an der Washington University School of Medicine, Division Hematology/Oncology, St. Louis, MO, USA (1992-1995).
PostDoc am Biozentrum in der Abteilung Biochemie der Universität Basel (1996).
Prof. Dr. Max Cloetta Stipendiat am Friedrich Miescher Institut der Novartis Forschungsstiftung (1997-2002).
Oberassistent am Physiologischen Institut der Universität Zürich (2002-2008).

Beruflicher Werdegang

1997-2002 Aufbau einer eigenen Forschungsgruppe als Prof. Dr. Max Cloetta Stipendiat am Friedrich Miescher Institut der Novartis Forschungsstiftung.
1999 Privatdozent der Universität Basel auf dem Gebiet Zellbiologie.
2004 Privatdozent der Universität Zürich für Physiologie und Zellbiologie.
2007 Titularprofessor der Universität Zürich.
2008 Professor in der Abteilung Biotechnologie der Zürcher Hochschule für Angewandte Wissenschaften

Mitglied in Netzwerken

• Life Sciences Switzerland
• American Society for Cell Biology
• Xing
• Linkedin
• Biotechnet

Projekte

• Bestenförderung von Lernenden / ProjektleiterIn / Projekt laufend
• Forschung und Entwicklung Zellphysiologie / ProjektleiterIn / Projekt abgeschlossen
• A platform enabling efficient and effective delivery and packaging of therapeutic molecules / ProjektleiterIn / Projekt abgeschlossen
• Novel cell-based immunization technology and functional validation of resulting antibodies / ProjektleiterIn / Projekt abgeschlossen
• Antibody discovery for therapy of pain / ProjektleiterIn / Projekt abgeschlossen

Publikationen

• Sabani, Besmira; Brand, Michael; Albert, Ina; Inderbitzin, Joelle; Eichenseher, Fritz; Schmelcher, Mathias; Rohrer, Jack; Riedl, Rainer; Lehmann, Steffi,

  2023.

  A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging.

  Nanomedicine: Nanotechnology, Biology and Medicine.

  47(102607).

  Verfügbar unter: https://doi.org/10.1016/j.nano.2022.102607

• Kozono, Takuma; Sato, Hiroyuki; Okumura, Wataru; Jogano, Chifuyu; Tamura-Nakano, Miwa; Kawamura, Yuki I.; Rohrer, Jack; Tonozuka, Takashi; Nishikawa, Atsushi,

  2021.

  The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region.

  Scientific Reports.

  11(753).

  Verfügbar unter: https://doi.org/10.1038/s41598-020-80258-5

• Luginbühl, Vera; Meier, Nicolas; Kovar, Karin; Rohrer, Jack,

  2018.

  Intracellular drug delivery : potential usefulness of engineered Shiga toxin subunit B for targeted cancer therapy.

  Biotechnology Advances.

  36(3), S. 613-623.

  Verfügbar unter: https://doi.org/10.1016/j.biotechadv.2018.02.005

• Peanne, Romain; Legrand, Dominique; Duvet, Sandrine; Mir, Anne-Marie; Matthijs, Gert; Rohrer, Jack; Foulquier, Francois,

  2011.

  Differential effects of lobe A and lobe B of the conserved oligomeric golgi complex on the stability of β1,4-galactosyltransferase 1 and α2,6-sialyltransferase 1.

  Glycobiology.

  21(7), S. 864-876.

  Verfügbar unter: https://doi.org/10.1093/glycob/cwq176

• Schaub, Beat E.; Berger, Eric G.; Rohrer, Jack,

  2008.

  Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition.

  Cellular and Molecular Life Sciences.

  65(22), S. 3677-3687.

  Verfügbar unter: https://doi.org/10.1007/s00018-008-8446-y

• Schaub, Beat E.; Berger, Bea; Berger, Eric G.; Rohrer, Jack,

  2006.

  Transition of galactosyltransferase 1 from trans-golgi cisterna to the trans-golgi network is signal mediated.

  Molecular Biology of the Cell.

  17(12), S. 5153-5162.

  Verfügbar unter: https://doi.org/10.1091/mbc.e06-08-0665

• Nair, Prashant; Schaub, Beat E.; Huang, Kai; Chen, Xiang; Murphy, Robert F.; Griffith, Janice M.; Geuze, Hans J.; Rohrer, Jack,

  2005.

  Characterization of the TGN exit signal of the human mannose 6-phosphate uncovering enzyme.

  Journal of Cell Science.

  118(13), S. 2949-2956.

  Verfügbar unter: https://doi.org/10.1242/jcs.02434

• Pulipparacharuvil, Suprabha; Akbar, Mohammed; Ray, Sanchali; Sevrioukov, Evgueny A.; Haberman, Adam S.; Rohrer, Jack; Krämer, Helmut,

  2005.

  Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules.

  Journal of Cell Science.

  118(16), S. 3663-3673.

  Verfügbar unter: https://doi.org/10.1242/jcs.02502

• Müntener, Kathrin; Zwicky, Roman; Csucs, Gabor; Rohrer, Jack; Baici, Antonio,

  2004.

  Exon skipping of cathepsin B : mitochondrial targeting of a lysosomal peptidase provokes cell death.

  Journal of Biological Chemistry.

  279(39), S. 41012-41017.

  Verfügbar unter: https://doi.org/10.1074/jbc.M405333200

• Stöckli, Jacqueline; Höning, Stefan; Rohrer, Jack,

  2004.

  The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding.

  Journal of Biological Chemistry.

  279(22), S. 23542-23549.

  Verfügbar unter: https://doi.org/10.1074/jbc.M313525200

• Stöckli, Jacqueline; Rohrer, Jack,

  2004.

  The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes.

  Molecular Biology of the Cell.

  15(6), S. 2617-2626.

  Verfügbar unter: https://doi.org/10.1091/mbc.e03-11-0808

• Nair, Prashant; Schaub, Beat E; Rohrer, Jack,

  2003.

  Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor.

  Journal of Biological Chemistry.

  278(27), S. 24753-24758.

  Verfügbar unter: https://doi.org/10.1074/jbc.M300174200

• Berger, Eric G.; Rohrer, Jack,

  2003.

  Galactosyltransferase — still up and running.

  Biochimie.

  85(3-4), S. 261-274.

  Verfügbar unter: https://doi.org/10.1016/S0300-9084(03)00008-7

• Crottet, Pascal; Meyer, Daniel M.; Rohrer, Jack; Spiess, Martin,

  2002.

  ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes.

  Molecular Biology of the Cell.

  13(10), S. 3672-3682.

  Verfügbar unter: https://doi.org/10.1091/mbc.e02-05-0309

• Mathews, Paul M.; Guerra, Carolyn B.; Jiang, Ying; Grbovic, Olivera M.; Kao, Benjamin H.; Schmidt, Stephen D.; Dinakar, Ravi; Mercken, Marc; Hille-Rehfeld, Annette; Rohrer, Jack; Mehta, Pankaj; Cataldo, Anne M.; Nixon, Ralph A.,

  2002.

  Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Aβ secretion : role for altered lysosomal hydrolase distribution in β-amyloidogenesis.

  Journal of Biological Chemistry.

  277(7), S. 5299-5307.

  Verfügbar unter: https://doi.org/10.1074/jbc.M108161200

• Lee, Wang-Sik; Rohrer, Jack; Kornfeld, Rosalind; Kornfeld, Stuart,

  2002.

  Multiple signals regulate trafficking of the mannose 6-phosphate-uncovering enzyme.

  Journal of Biological Chemistry.

  277(5), S. 3544-3551.

  Verfügbar unter: https://doi.org/10.1074/jbc.M108531200

• Rohrer, Jack; Kornfeld, Rosalind,

  2001.

  Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network.

  Molecular Biology of the Cell.

  12(6), S. 1623-1631.

  Verfügbar unter: https://doi.org/10.1091/mbc.12.6.1623

• Schweizer, Anja; Stahl, Philip D.; Rohrer, Jack,

  2000.

  A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting.

  Journal of Biological Chemistry.

  275(38), S. 29694-29700.

  Verfügbar unter: https://doi.org/10.1074/jbc.M000571200

• Schweizer, Anja; Valdenaire, Olivier; Köster, Anja; Lang, Yolande; Schmitt, Georg; Lenz, Barbara; Bluethmann, Horst; Rohrer, Jack,

  1999.

  Neonatal lethality in mice deficient in XCE, a novel member of the endothelin-converting enzyme and neutral endopeptidase family.

  Journal of Biological Chemistry.

  274(29), S. 20450-20456.

  Verfügbar unter: https://doi.org/10.1074/jbc.274.29.20450

• Schweizer, Anja; Löffler, Bernd-Michael; Rohrer, Jack,

  1999.

  Palmitoylation of the three isoforms of human endothelin-converting enzyme-1.

  Biochemical Journal.

  340(3), S. 649-656.

  Verfügbar unter: https://doi.org/10.1042/bj3400649

• Olayioye, Monilola A.; Graus-Porta, Diana; Beerli, Roger R.; Rohrer, Jack; Gay, Brigitte; Hynes, Nancy E.,

  1998.

  ErbB-1 and ErbB-2 acquire distinct signaling properties dependent upon their dimerization partner.

  Molecular and Cellular Biology.

  18(9), S. 5042-5051.

  Verfügbar unter: https://doi.org/10.1128/MCB.18.9.5042

• Schweizer, Anja; Kornfeld, Stuart; Rohrer, Jack,

  1997.

  Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail.

  Proceedings of the National Academy of Sciences of the United States of America.

  94(26), S. 14471-14476.

  Verfügbar unter: https://doi.org/10.1073/pnas.94.26.14471

• Hicke, Linda; Zanolari, Bettina; Pypaert, Marc; Rohrer, Jack; Riezman, Howard,

  1997.

  Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein.

  Molecular Biology of the Cell.

  8(1), S. 13-31.

  Verfügbar unter: https://doi.org/10.1091/mbc.8.1.13

• Schweizer, Anja; Kornfeld, Stuart; Rohrer, Jack,

  1996.

  Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting.

  Journal of Cell Biology.

  132(4), S. 577-584.

  Verfügbar unter: https://doi.org/10.1083/jcb.132.4.577

• Rohrer, Jack; Schweizer, Anja; Russel, David; Kornfeld, Stuart,

  1996.

  The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.

  Journal of Cell Biology.

  132(4), S. 565-576.

  Verfügbar unter: https://doi.org/10.1083/jcb.132.4.565

• Rohrer, Jack; Schweizer, Anja; Johnson, Karl F.; Kornfeld, Stuart,

  1995.

  A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes.

  Journal of Cell Biology.

  130(6), S. 1297-1306.

  Verfügbar unter: https://doi.org/10.1083/jcb.130.6.1297

• Schweizer, Anja; Rohrer, Jack; Kornfeld, Stuart,

  1995.

  Determination of the structural requirements for palmitoylation of p63.

  Journal of Biological Chemistry.

  270(16), S. 9638-9644.

  Verfügbar unter: https://doi.org/10.1074/jbc.270.16.9638

• Schweizer, Anja; Rohrer, Jack; Slot, Jan W.; Geuze, Hans J.; Kornfeld, Stuart,

  1995.

  Reassessment of the subcellular localization of p63.

  Journal of Cell Science.

  108, S. 2477-2485.

  Verfügbar unter: http://jcs.biologists.org/content/108/6/2477

• Schweizer, Anja; Rohrer, Jack; Hauri, Hanspeter; Kornfeld, Stuart,

  1994.

  Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers.

  Journal of Cell Biology.

  126(1), S. 25-39.

  Verfügbar unter: https://doi.org/10.1083/jcb.126.1.25

• Rohrer, Jack; Bénédetti, Hélène; Zanolari, Bettina; Riezman, Howard,

  1993.

  Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

  Molecular Biology of the Cell.

  4(5), S. 511-521.

  Verfügbar unter: https://doi.org/10.1091/mbc.4.5.511

• Schweizer, Anja; Rohrer, Jack; Jenö, Paul; DeMaio, Antonio; Buchman, Timothy G.; Hauri, Hans-Peter,

  1993.

  A reversibly palmitoylated resident protein (p63) of an ER-golgi intermediate compartment is related to a circulatory shock resuscitation protein.

  Journal of Cell Science.

  104, S. 685-694.

  Verfügbar unter: http://jcs.biologists.org/content/104/3/685.short

• Raths, Susan; Rohrer, Jack; Crausaz, Fabienne; Riezman, Howard,

  1993.

  end3 and end4 : two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.

  Journal of Cell Biology.

  120(1), S. 55-65.

  Verfügbar unter: https://doi.org/10.1083/jcb.120.1.55

• Rohrer, Jack; Kuhn, Andreas,

  1990.

  The function of a leader peptide in translocating charged amino acyl residues across a membrane.

  Science.

  250(4986), S. 1418-1421.

  Verfügbar unter: https://doi.org/10.1126/science.2124001

• Kuhn, Andreas; Rohrer, Jack; Gallusser, Andreas,

  1990.

  Bacteriophages M13 and Pf3 tell us how proteins insert into the membrane.

  Journal of Structural Biology.

  104(1-3), S. 38-43.

  Verfügbar unter: https://doi.org/10.1016/1047-8477(90)90055-H

• Rimann, Markus; Mathes, Stephanie; Raghunath, Michael; Rohrer, Jack,

  2016.

  Zurich university of applied sciences : center for cell biology and tissue engineering [Poster].

  In:

  Roche Symposium, Basel, 2016.

• Raghunath, Michael; Rimann, Markus; Mathes, Stephanie; Rohrer, Jack,

  2016.

  Zurich University of Applied Sciences : center for cell biology and tissue engineering [Poster].

  In:

  Roche Symposium, Basel, 2016.

• Schaub, Beat E; Nair, Prashant; Rohrer, Jack,

  2005.

  Analysis of protein transport to lysosomes.

  Current Protocols in Cell Biology.

  27(1).

  Verfügbar unter: https://doi.org/10.1002/0471143030.cb1508s27

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