Prof. Dr. Jack Rohrer

Personal profile

Management role

Head, Cell Physiology and Cellular Engineering Group

Position at the ZHAW

Head of the group Cell Physiology and Cellular Engineering, special expertise in: glycobiology, recombinant protein expression, development of cell based assays, induced pluripotent stem cells, subcellular drug delivery, lysosomal biogenesis and molecular mechanisms of intracellular protein trafficking in general.

www.zhaw.ch/de/lsfm/institute-zentren/icbt/zellbiologie-und-tissue-engineering/zellphysiologie-und-zell-engineering/

Expertise and research interests

Specific technical know-how covers microscopy (including confocal microscopy and life imaging) as well as a multitude of methods from molecular biology, biochemistry, cell biology and immunology. The main research subjects include induced pluripotent stem cells, development of novel antibodies against complex proteins, the development of cell based assays, and subcellular drug delivery.

Educational background

Study of Biology II at the University of Basel (1984-1988).
PhD thesis at the Department of Biochemistry, Biozentrum of the University of Basel (1988-1992).
PostDoc at the Division Hematology/Oncology, Washington University School of Medicine, St Louis, USA (1992-1995).
PostDoc at the Department of Biochemistry, Biozentrum of the University of Basel (1996).
Prof. Dr. Max Cloëtta-Fellow at the Friedrich Miescher Institute, Novartis Research Foundation, Basel (1997-2002).
'Oberassistent' at the Institute of Physiology, University of Zürich (2002-2008).

Professional milestones

1997-2002 Establishing my own research group as a Prof. Dr. Max Cloetta fellow at the Friedrich Miescher Institute of the Novartis Research Foundation.
1999 Appointed by the Faculty of Natural Sciences of the University of Basel as 'Privatdozent' for Cell Biology.
2004 Appointed by the Medical Faculty of the University of Zürich as 'Privatdozent' for Physiology and Cell Biology.
2007 Appointed by the Medical Faculty of the University of Zürich as 'Titularprofessor'
2008 Professor of the Section Biotechnology of the Zurich University of Applied Sciences

Membership of networks

• Life Sciences Switzerland
• American Society for Cell Biology
• Xing
• Linkedin
• Biotechnet

Projects

• Bestenförderung von Lernenden / Project leader / Project ongoing
• Forschung und Entwicklung Zellphysiologie / Project leader / Project completed
• A platform enabling efficient and effective delivery and packaging of therapeutic molecules / Project leader / Project completed
• Novel cell-based immunization technology and functional validation of resulting antibodies / Project leader / Project completed
• Antibody discovery for therapy of pain / Project leader / Project completed

Publications

• Sabani, Besmira; Brand, Michael; Albert, Ina; Inderbitzin, Joelle; Eichenseher, Fritz; Schmelcher, Mathias; Rohrer, Jack; Riedl, Rainer; Lehmann, Steffi,

  2023.

  A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging.

  Nanomedicine: Nanotechnology, Biology and Medicine.

  47(102607).

  Available from: https://doi.org/10.1016/j.nano.2022.102607

• Kozono, Takuma; Sato, Hiroyuki; Okumura, Wataru; Jogano, Chifuyu; Tamura-Nakano, Miwa; Kawamura, Yuki I.; Rohrer, Jack; Tonozuka, Takashi; Nishikawa, Atsushi,

  2021.

  The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region.

  Scientific Reports.

  11(753).

  Available from: https://doi.org/10.1038/s41598-020-80258-5

• Luginbühl, Vera; Meier, Nicolas; Kovar, Karin; Rohrer, Jack,

  2018.

  Intracellular drug delivery : potential usefulness of engineered Shiga toxin subunit B for targeted cancer therapy.

  Biotechnology Advances.

  36(3), pp. 613-623.

  Available from: https://doi.org/10.1016/j.biotechadv.2018.02.005

• Peanne, Romain; Legrand, Dominique; Duvet, Sandrine; Mir, Anne-Marie; Matthijs, Gert; Rohrer, Jack; Foulquier, Francois,

  2011.

  Differential effects of lobe A and lobe B of the conserved oligomeric golgi complex on the stability of β1,4-galactosyltransferase 1 and α2,6-sialyltransferase 1.

  Glycobiology.

  21(7), pp. 864-876.

  Available from: https://doi.org/10.1093/glycob/cwq176

• Schaub, Beat E.; Berger, Eric G.; Rohrer, Jack,

  2008.

  Dissection of a novel molecular determinant mediating golgi to trans-golgi network transition.

  Cellular and Molecular Life Sciences.

  65(22), pp. 3677-3687.

  Available from: https://doi.org/10.1007/s00018-008-8446-y

• Schaub, Beat E.; Berger, Bea; Berger, Eric G.; Rohrer, Jack,

  2006.

  Transition of galactosyltransferase 1 from trans-golgi cisterna to the trans-golgi network is signal mediated.

  Molecular Biology of the Cell.

  17(12), pp. 5153-5162.

  Available from: https://doi.org/10.1091/mbc.e06-08-0665

• Nair, Prashant; Schaub, Beat E.; Huang, Kai; Chen, Xiang; Murphy, Robert F.; Griffith, Janice M.; Geuze, Hans J.; Rohrer, Jack,

  2005.

  Characterization of the TGN exit signal of the human mannose 6-phosphate uncovering enzyme.

  Journal of Cell Science.

  118(13), pp. 2949-2956.

  Available from: https://doi.org/10.1242/jcs.02434

• Pulipparacharuvil, Suprabha; Akbar, Mohammed; Ray, Sanchali; Sevrioukov, Evgueny A.; Haberman, Adam S.; Rohrer, Jack; Krämer, Helmut,

  2005.

  Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules.

  Journal of Cell Science.

  118(16), pp. 3663-3673.

  Available from: https://doi.org/10.1242/jcs.02502

• Müntener, Kathrin; Zwicky, Roman; Csucs, Gabor; Rohrer, Jack; Baici, Antonio,

  2004.

  Exon skipping of cathepsin B : mitochondrial targeting of a lysosomal peptidase provokes cell death.

  Journal of Biological Chemistry.

  279(39), pp. 41012-41017.

  Available from: https://doi.org/10.1074/jbc.M405333200

• Stöckli, Jacqueline; Höning, Stefan; Rohrer, Jack,

  2004.

  The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding.

  Journal of Biological Chemistry.

  279(22), pp. 23542-23549.

  Available from: https://doi.org/10.1074/jbc.M313525200

• Stöckli, Jacqueline; Rohrer, Jack,

  2004.

  The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes.

  Molecular Biology of the Cell.

  15(6), pp. 2617-2626.

  Available from: https://doi.org/10.1091/mbc.e03-11-0808

• Nair, Prashant; Schaub, Beat E; Rohrer, Jack,

  2003.

  Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor.

  Journal of Biological Chemistry.

  278(27), pp. 24753-24758.

  Available from: https://doi.org/10.1074/jbc.M300174200

• Berger, Eric G.; Rohrer, Jack,

  2003.

  Galactosyltransferase — still up and running.

  Biochimie.

  85(3-4), pp. 261-274.

  Available from: https://doi.org/10.1016/S0300-9084(03)00008-7

• Crottet, Pascal; Meyer, Daniel M.; Rohrer, Jack; Spiess, Martin,

  2002.

  ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes.

  Molecular Biology of the Cell.

  13(10), pp. 3672-3682.

  Available from: https://doi.org/10.1091/mbc.e02-05-0309

• Mathews, Paul M.; Guerra, Carolyn B.; Jiang, Ying; Grbovic, Olivera M.; Kao, Benjamin H.; Schmidt, Stephen D.; Dinakar, Ravi; Mercken, Marc; Hille-Rehfeld, Annette; Rohrer, Jack; Mehta, Pankaj; Cataldo, Anne M.; Nixon, Ralph A.,

  2002.

  Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Aβ secretion : role for altered lysosomal hydrolase distribution in β-amyloidogenesis.

  Journal of Biological Chemistry.

  277(7), pp. 5299-5307.

  Available from: https://doi.org/10.1074/jbc.M108161200

• Lee, Wang-Sik; Rohrer, Jack; Kornfeld, Rosalind; Kornfeld, Stuart,

  2002.

  Multiple signals regulate trafficking of the mannose 6-phosphate-uncovering enzyme.

  Journal of Biological Chemistry.

  277(5), pp. 3544-3551.

  Available from: https://doi.org/10.1074/jbc.M108531200

• Rohrer, Jack; Kornfeld, Rosalind,

  2001.

  Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network.

  Molecular Biology of the Cell.

  12(6), pp. 1623-1631.

  Available from: https://doi.org/10.1091/mbc.12.6.1623

• Schweizer, Anja; Stahl, Philip D.; Rohrer, Jack,

  2000.

  A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting.

  Journal of Biological Chemistry.

  275(38), pp. 29694-29700.

  Available from: https://doi.org/10.1074/jbc.M000571200

• Schweizer, Anja; Valdenaire, Olivier; Köster, Anja; Lang, Yolande; Schmitt, Georg; Lenz, Barbara; Bluethmann, Horst; Rohrer, Jack,

  1999.

  Neonatal lethality in mice deficient in XCE, a novel member of the endothelin-converting enzyme and neutral endopeptidase family.

  Journal of Biological Chemistry.

  274(29), pp. 20450-20456.

  Available from: https://doi.org/10.1074/jbc.274.29.20450

• Schweizer, Anja; Löffler, Bernd-Michael; Rohrer, Jack,

  1999.

  Palmitoylation of the three isoforms of human endothelin-converting enzyme-1.

  Biochemical Journal.

  340(3), pp. 649-656.

  Available from: https://doi.org/10.1042/bj3400649

• Olayioye, Monilola A.; Graus-Porta, Diana; Beerli, Roger R.; Rohrer, Jack; Gay, Brigitte; Hynes, Nancy E.,

  1998.

  ErbB-1 and ErbB-2 acquire distinct signaling properties dependent upon their dimerization partner.

  Molecular and Cellular Biology.

  18(9), pp. 5042-5051.

  Available from: https://doi.org/10.1128/MCB.18.9.5042

• Schweizer, Anja; Kornfeld, Stuart; Rohrer, Jack,

  1997.

  Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail.

  Proceedings of the National Academy of Sciences of the United States of America.

  94(26), pp. 14471-14476.

  Available from: https://doi.org/10.1073/pnas.94.26.14471

• Hicke, Linda; Zanolari, Bettina; Pypaert, Marc; Rohrer, Jack; Riezman, Howard,

  1997.

  Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein.

  Molecular Biology of the Cell.

  8(1), pp. 13-31.

  Available from: https://doi.org/10.1091/mbc.8.1.13

• Schweizer, Anja; Kornfeld, Stuart; Rohrer, Jack,

  1996.

  Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting.

  Journal of Cell Biology.

  132(4), pp. 577-584.

  Available from: https://doi.org/10.1083/jcb.132.4.577

• Rohrer, Jack; Schweizer, Anja; Russel, David; Kornfeld, Stuart,

  1996.

  The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane.

  Journal of Cell Biology.

  132(4), pp. 565-576.

  Available from: https://doi.org/10.1083/jcb.132.4.565

• Rohrer, Jack; Schweizer, Anja; Johnson, Karl F.; Kornfeld, Stuart,

  1995.

  A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes.

  Journal of Cell Biology.

  130(6), pp. 1297-1306.

  Available from: https://doi.org/10.1083/jcb.130.6.1297

• Schweizer, Anja; Rohrer, Jack; Kornfeld, Stuart,

  1995.

  Determination of the structural requirements for palmitoylation of p63.

  Journal of Biological Chemistry.

  270(16), pp. 9638-9644.

  Available from: https://doi.org/10.1074/jbc.270.16.9638

• Schweizer, Anja; Rohrer, Jack; Slot, Jan W.; Geuze, Hans J.; Kornfeld, Stuart,

  1995.

  Reassessment of the subcellular localization of p63.

  Journal of Cell Science.

  108, pp. 2477-2485.

  Available from: http://jcs.biologists.org/content/108/6/2477

• Schweizer, Anja; Rohrer, Jack; Hauri, Hanspeter; Kornfeld, Stuart,

  1994.

  Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers.

  Journal of Cell Biology.

  126(1), pp. 25-39.

  Available from: https://doi.org/10.1083/jcb.126.1.25

• Rohrer, Jack; Bénédetti, Hélène; Zanolari, Bettina; Riezman, Howard,

  1993.

  Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast.

  Molecular Biology of the Cell.

  4(5), pp. 511-521.

  Available from: https://doi.org/10.1091/mbc.4.5.511

• Schweizer, Anja; Rohrer, Jack; Jenö, Paul; DeMaio, Antonio; Buchman, Timothy G.; Hauri, Hans-Peter,

  1993.

  A reversibly palmitoylated resident protein (p63) of an ER-golgi intermediate compartment is related to a circulatory shock resuscitation protein.

  Journal of Cell Science.

  104, pp. 685-694.

  Available from: http://jcs.biologists.org/content/104/3/685.short

• Raths, Susan; Rohrer, Jack; Crausaz, Fabienne; Riezman, Howard,

  1993.

  end3 and end4 : two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.

  Journal of Cell Biology.

  120(1), pp. 55-65.

  Available from: https://doi.org/10.1083/jcb.120.1.55

• Rohrer, Jack; Kuhn, Andreas,

  1990.

  The function of a leader peptide in translocating charged amino acyl residues across a membrane.

  Science.

  250(4986), pp. 1418-1421.

  Available from: https://doi.org/10.1126/science.2124001

• Kuhn, Andreas; Rohrer, Jack; Gallusser, Andreas,

  1990.

  Bacteriophages M13 and Pf3 tell us how proteins insert into the membrane.

  Journal of Structural Biology.

  104(1-3), pp. 38-43.

  Available from: https://doi.org/10.1016/1047-8477(90)90055-H

• Rimann, Markus; Mathes, Stephanie; Raghunath, Michael; Rohrer, Jack,

  2016.

  Zurich university of applied sciences : center for cell biology and tissue engineering [poster].

  In:

  Roche Symposium, Basel, 2016.

• Raghunath, Michael; Rimann, Markus; Mathes, Stephanie; Rohrer, Jack,

  2016.

  Zurich University of Applied Sciences : center for cell biology and tissue engineering [poster].

  In:

  Roche Symposium, Basel, 2016.

• Schaub, Beat E; Nair, Prashant; Rohrer, Jack,

  2005.

  Analysis of protein transport to lysosomes.

  Current Protocols in Cell Biology.

  27(1).

  Available from: https://doi.org/10.1002/0471143030.cb1508s27

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