Prof. Dr. Michael Raghunath

Prof. Dr. Michael Raghunath

ZHAW Life Sciences und Facility Management
Fachgruppe Tissue Engineering
Einsiedlerstrasse 31
8820 Wädenswil

+41 58 934 55 18
michael.raghunath@zhaw.ch

Projekte

Publikationen

Beiträge in wissenschaftlicher Zeitschrift, peer-reviewed

Veneruso, V. et al. (2026) 'Engineering a multilayered thin-film agarose-based hydrogel to support adipose-derived stromal vascular fraction therapy in spinal cord injury', International Journal of Biological Macromolecules, 370(152683). doi: 10.1016/j.ijbiomac.2026.152683.
Wan, H.-Y. et al. (2023) 'Stabilization and improved functionality of three-dimensional perfusable microvascular networks in microfluidic devices under macromolecular crowding', Biomaterials Research, 27(32). doi: 10.1186/s40824-023-00375-w.
Vo, A. N. et al. (2022) 'Enhancement of neuroglial extracellular matrix formation and physiological activity of dopaminergic neural cocultures by macromolecular crowding', Cells, 11(14), p. 2131. doi: 10.3390/cells11142131.
Pinelli, F. et al. (2022) 'Biomaterial-mediated factor delivery for spinal cord injury treatment', Biomedicines, 10(7), p. 1673. doi: 10.3390/biomedicines10071673.
Später, T. et al. (2022) 'Engineering microparticles based on solidified stem cell secretome with an augmented pro-angiogenic factor portfolio for therapeutic angiogenesis', Bioactive Materials, (17), pp. 526–541. doi: 10.1016/j.bioactmat.2022.03.015.
D'Agostino, S. et al. (2022) 'Macromolecular crowding tuned extracellular matrix deposition in a bioprinted human rhabdomyosarcoma model', Bioprinting, 27(e00213). doi: 10.1016/j.bprint.2022.e00213.
Rampin, A. et al. (2022) 'Allogeneic serum and macromolecular crowding maintain native equine tenocyte function in culture', Cells, 11(9), p. 1562. doi: 10.3390/cells11091562.
Raghunath, M. and Zeugolis, D. I. (2021) 'Transforming eukaryotic cell culture with macromolecular crowding', Trends in Biochemical Sciences, 46(10), pp. 805–811. doi: 10.1016/j.tibs.2021.04.006.
Tsiapalis, D. et al. (2020) 'The synergistic effect of low oxygen tension and macromolecular crowding in the development of extracellular matrix-rich tendon equivalents', Biofabrication, 12(2), p. 25018. doi: 10.1088/1758-5090/ab6412.
Assunção, M. et al. (2020) 'Macromolecular dextran sulfate facilitates extracellular matrix deposition by electrostatic interaction independent from a macromolecular crowding effect', Materials Science and Engineering C: Materials for Biological Applications, 106(110280). doi: 10.1016/j.msec.2019.110280.
Fernández-Majada, V. et al. (2020) 'Editorial : when the shape does matter : three-dimensional in vitro models of epithelial barriers', Frontiers in Bioengineering and Biotechnology, 8(617361). doi: 10.3389/fbioe.2020.617361.
Ling, L. et al. (2020) 'Enhancing the efficacy of stem cell therapy with glycosaminoglycans', Stem Cell Reports, 14(1), pp. 105–121. doi: 10.1016/j.stemcr.2019.12.003.
Kopanska, K. S. et al. (2019) 'Advanced in vitro models analysis', ALTEX - Alternatives to Animal Experimentation, 36(1), pp. 144–147. doi: 10.14573/altex.1812131.
Kremer, A. et al. (2019) 'Ciclopirox olamine promotes the angiogenic response of endothelial cells and mesenchymal stem cells', Clinical Hemorheology and Microcirculation, 73(2), pp. 317–328. doi: 10.3233/CH-190559.
Wong, C.-W. et al. (2019) 'In vitro expansion of keratinocytes on human dermal fibroblast-derived matrix retains their stem-like characteristics', Scientific Reports, 9(1), p. 18561. doi: 10.1038/s41598-019-54793-9.
Graham, J., Raghunath, M. and Vogel, V. (2019) 'Fibrillar fibronectin plays a key role as nucleator of collagen I polymerization during macromolecular crowding-enhanced matrix assembly', Biomaterials Science, 7(11), pp. 4519–4535. doi: 10.1039/C9BM00868C.
Lo, L. M., Raghunath, M. and Lee, K. K. H. (2019) 'Growing human dermal fibroblasts as spheroids renders them susceptible for early expression of pluripotency genes', Advanced Biosystems, 3(10). doi: 10.1002/adbi.201900094.
Gaspar, D. et al. (2019) 'Local pharmacological induction of angiogenesis : drugs for cells and cells as drugs', Advanced Drug Delivery Reviews, 146, pp. 126–154. doi: 10.1016/j.addr.2019.06.002.
Bertlein, S. et al. (2019) 'Permanent hydrophilization and generic bioactivation of melt electrowritten scaffolds', Advanced Healthcare Materials, 8(7). doi: 10.1002/adhm.201801544.
Coentro, J. Q. et al. (2018) 'Current and upcoming therapies to modulate skin scarring and fibrosis', Advanced Drug Delivery Reviews. doi: 10.1016/j.addr.2018.08.009.
Pugliese, E. et al. (2018) 'Wound healing and scar wars', Advanced Drug Delivery Reviews, 129. doi: 10.1016/j.addr.2018.05.010.
Sorushanova, A. et al. (2018) 'The collagen suprafamily : from biosynthesis to advanced biomaterial development', Advanced Materials. doi: 10.1002/adma.201801651.
Raghunath, M. et al. (2018) 'TEDD annual meeting with 3D bioprinting workshop', Chimia, 72(1/2), pp. 76–79. doi: 10.2533/chimia.2018.76.
Blocki, A. et al. (2018) 'The controversial origin of pericytes during angiogenesis : implications for cell-based therapeutic angiogenesis and cell-based therapies', Clinical Hemorheology and Microcirculation, 69(1-2), pp. 215–232. doi: 10.3233/CH-189132.
Arai, S. et al. (2018) 'RGB‐Color intensiometric indicators to visualize spatiotemporal dynamics of ATP in single cells', Angewandte Chemie: International Edition, 57(34), pp. 10873–10878. doi: 10.1002/anie.201804304.
Samsonraj, R. M. et al. (2017) 'Concise review : multifaceted characterization of human mesenchymal stem cells for use in regenerative medicine', Stem Cells Translational Medicine, 6(12), pp. 2173–2185. doi: 10.1002/sctm.17-0129.
Patrikoski, M. et al. (2017) 'Effects of macromolecular crowding on human adipose stem cell culture in fetal bovine serum, human serum and defined xeno-free/serum-free conditions', Stem Cells International, 2017(6909163). doi: 10.1155/2017/6909163.
Kriszt, R. et al. (2017) 'Optical visualisation of thermogenesis in stimulated singlecell brown adipocytes', Scientific Reports. doi: 10.1038/s41598-017-00291-9.
Hou, Y. et al. (2017) 'Ca2+-associated triphasic pH changes in mitochondria during brown adipocyte activation', Molecular Metabolism, 6(8), pp. 797–808. doi: 10.1016/j.molmet.2017.05.013.
Goralczyk, A. et al. (2017) 'TRP channels in brown and white adipogenesis from human progenitors : new therapeutic targets and the caveats associated with the common antibiotic, streptomycin', The FASEB Journal, 31(8). doi: 10.1096/fj.201601081RR.
Lim, N. S. J. et al. (2016) 'Combination of ciclopirox olamine and sphingosine-1-phosphate as granulation enhancer in diabetic wounds', Wound Repair and Regeneration, 24(5), pp. 795–809. doi: 10.1111/wrr.12463.
H. Lee, M. et al. (2016) 'ECM microenvironment unlocks brown adipogenic potential of adult human bone marrow-derived MSCs', Scientific Reports, 6(21173). doi: 10.1038/srep21173.
Benny, P. et al. (2016) 'Improving 2D and 3D skin in vitro models, using macromolecular crowding', Journal of Visualized Experiments, 2016(114). doi: 10.3791/53642.
Benny, P. et al. (2015) 'Making more matrix : enhancing the deposition of dermal-epidermal junction components in vitro and accelerating organotypic skin culture development, using macromolecular crowding', Tissue Engineering - Part A, 21(1-2). doi: 10.1089/ten.TEA.2013.0784.
Kumar, P. et al. (2015) 'Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies', Scientific Reports, 5(8729). doi: 10.1038/srep08729.
Blocki, A. et al. (2015) 'Microcapsules engineered to support mesenchymal stem cell (MSC) survival and proliferation enable long-term retention of MSCs in infarcted myocardium', Biomaterials, 53, pp. 12–24. doi: 10.1016/j.biomaterials.2015.02.075.
Sham, A. et al. (2015) 'Incorporation of a prolyl hydroxylase inhibitor into scaffolds : a strategy for stimulating vascularization', Tissue Engineering - Part A, 21(5-6). doi: 10.1089/ten.TEA.2014.0077.
Movahednia, M. M. et al. (2015) 'Differential effects of the extracellular microenvironment on human embryonic stem cells differentiation into keratinocytes and their subsequent replicative lifespan', Tissue Engineering - Part A, 21(7-8). doi: 10.1089/ten.TEA.2014.0551.
Samsonraj, R. M. et al. (2015) 'Establishing criteria for human mesenchymal stem cell potency', Stem Cells, 33(6), pp. 1878–1891. doi: 10.1002/stem.1982.
Kumar, P. et al. (2015) 'Accelerated development of supramolecular corneal stromal-like assemblies from corneal fibroblasts in the presence of macromolecular crowders', Tissue Engineering - Part C: Methods, 21(7). doi: 10.1089/ten.TEC.2014.0387.
Peh, P. et al. (2015) 'Simultaneous delivery of highly diverse bioactive compounds from blend electrospun fibers for skin wound healing', Bioconjugate Chemistry, 26(7), pp. 1348–1358. doi: 10.1021/acs.bioconjchem.5b00123.
Blocki, A. et al. (2015) 'Sourcing of an alternative pericyte-like cell type from peripheral blood generated with pulsed macromolecular crowding', Molecular Therapy, 23(3), pp. 510–522. doi: 10.1038/mt.2014.232.
Rashid, R. et al. (2015) 'Macromolecular crowding gives rise to microviscosity, anomalous diffusion and accelerated actin polymerization', Physical Biology, 12(3), p. 034001. doi: 10.1088/1478-3975/12/3/034001.
Dewavrin, J.-Y. et al. (2015) 'Synergistic rate boosting of collagen fibrillogenesis in heterogeneous mixtures of crowding agents', Journal of Physical Chemistry B, 119(12), pp. 4350–4358. doi: 10.1021/jp5077559.
Rashid, R. et al. (2014) 'Mitochondrial routing of glucose and sucrose polymers after pinocytotic uptake : avenues for drug delivery', Biomacromolecules, 15(6), pp. 2119–2127. doi: 10.1021/bm500243m.
Dewavrin, J.-Y. et al. (2014) 'Tuning the architecture of three-dimensional collagen hydrogels by physiological macromolecular crowding', Acta Biomaterialia, 10(10), pp. 4351–4359. doi: 10.1016/j.actbio.2014.06.006.
Rashid, R. et al. (2014) 'Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation', Tissue Engineering - Part C: Methods, 20(12). doi: 10.1089/ten.tec.2013.0733.
Dewavrin, J.-Y. et al. (2014) 'Tuning the architecture of 3D collagen hydrogels by physiological macromolecular crowding', Acta Biomaterialia, 10(10), pp. 4351–4359. doi: 10.1016/j.actbio.2014.06.006.
Satyam, A. et al. (2014) 'Macromolecular crowding meets tissue engineering by self-assembly : a paradigm shift in regenerative medicine', Advanced Materials, 26(19), pp. 3024–3034. doi: 10.1002/adma.201304428.
Ang, X. M. et al. (2014) 'Macromolecular crowding amplifies adipogenesis of human bone marrow-derived MSCs by enhancing the pro-adipogenic microenvironment', Tissue Engineering - Part A, 20(5-6). doi: 10.1089/ten.tea.2013.0337.
Blocki, A. et al. (2013) 'Not all MSCs can act as pericytes : functional In vitro assays to distinguish pericytes from other mesenchymal stem cells in angiogenesis', Stem Cells and Development, 22(17). doi: 10.1089/scd.2012.0415.
Lim, S. H. et al. (2013) 'Complementary effects of prolyl hydroxylase inhibitors and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting', Integrative Biology, 2013(12), pp. 1474–1484. doi: 10.1039/C3IB40082D.
Tan, A. B.-S. et al. (2013) 'Cellular re- and de-programming by microenvironmental memory: why short TGFβ1 pulses can have long effects', Fibrogenesis & Tissue Repair, 6(12). doi: 10.1186/1755-1536-6-12.
Samsonraj, R. M. et al. (2013) 'Telomere length analysis of human mesenchymal stem cells by quantitative PCR', Gene, 519(2), pp. 348–355. doi: 10.1016/j.gene.2013.01.039.
Zeiger, A. S. et al. (2012) 'Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior', PLOS ONE, 7(5), p. e37904. doi: 10.1371/journal.pone.0037904.
Satyam, A. et al. (2012) 'In vitro evaluation of Ficoll‐enriched and genipin‐stabilised collagen scaffolds', Journal of Tissue Engineering and Regenerative Medicine, 8(3), pp. 233–241. doi: 10.1002/term.1522.
Chen, C. et al. (2011) 'Applying macromolecular crowding to enhance extracellular matrix deposition and its remodeling in vitro for tissue engineering and cell-based therapies', Advanced Drug Delivery Reviews, 63(4-5), pp. 277–290. doi: 10.1016/j.addr.2011.03.003.
Lareu, R. R. et al. (2010) 'Essential modification of the Sircol Collagen Assay for the accurate quantification of collagen content in complex protein solutions', Acta Biomaterialia, 6(8), pp. 3146–3151. doi: 10.1016/j.actbio.2010.02.004.
Raghunath, M. et al. (2009) 'Pharmacologically induced angiogenesis in transgenic zebrafish', Biochemical and Biophysical Research Communications, 378(4), pp. 766–771. doi: 10.1016/j.bbrc.2008.11.127.
Zeugolis, D. I. et al. (2009) 'An in situ and in vitro investigation for the transglutaminase potential in tissue engineering', Journal of Biomedical Materials Research, 92A(4), pp. 1310–1320. doi: 10.1002/jbm.a.32383.
Wang, Z. et al. (2009) 'Suberoylanilide hydroxamic acid : a potential epigenetic therapeutic agent for lung fibrosis?', The European Respiratory Journal, 34(1), pp. 145–155. doi: 10.1183/09031936.00084808.
Chen, C. Z. C. et al. (2009) 'The Scar‐in‐a‐Jar : studying potential antifibrotic compounds from the epigenetic to extracellular level in a single well', British Journal of Pharmacology, 158(5), pp. 1196–1209. doi: 10.1111/j.1476-5381.2009.00387.x.
Zeugolis, D. I. et al. (2008) 'Electro-spinning of pure collagen fibres : just an expensive way to make gelatin?', Biomaterials, 29(15), pp. 2293–2305. doi: 10.1016/j.biomaterials.2008.02.009.
Zeugolis, D. I. et al. (2008) 'Collagen solubility testing, a quality assurance step for reproducible electro-spun nano-fibre fabrication : a technical note', Journal of Biomaterials Science, Polymer Edition, 19(10), pp. 1307–1317. doi: 10.1163/156856208786052344.

Buchbeiträge, peer-reviewed

Stebler, S. and Raghunath, M. (2021) 'The scar-in-a-jar : in vitro fibrosis model for anti-fibrotic drug testing', in Hinz, B. and Lagares, D. (eds) Myofibroblasts. New York: Humana, pp. 147–156. doi: 10.1007/978-1-0716-1382-5_11.
Badowski, C. et al. (2018) 'Molecular Crowding – (in Cell Culture)', in Tissue engineering and regeneration : cell engineering and regeneration. Springer. doi: 10.1007/978-3-319-37076-7_50-1.
Coentro, J. Q. et al. (2017) 'Collagen quantification in tissue specimens', in Fibrosis. Springer. doi: 10.1007/978-1-4939-7113-8_22.

Schriftliche Konferenzbeiträge, peer-reviewed

Ling, L. et al. (2019) 'Biomimicry of glycosaminoglycans in the bone marrow microenvironment favour the expansion of highly potent human mesenchymal stem cells', in Cytotherapy : 25th Annual ISCT Meeting. Elsevier, p. S71. doi: 10.1016/j.jcyt.2019.03.463.

Weitere Publikationen

Moldovan, N. I., Moldovan, L. and Raghunath, M. (2019) 'Of balls, inks and cages : hybrid biofabrication of 3D tissue analogs', International Journal of Bioprinting, 5(1), p. 167. doi: 10.18063/ijb.v5i1.167.
Benny, P. and Raghunath, M. (2017) 'Making microenvironments : a look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications', Journal of Tissue Engineering. doi: 10.1177/2041731417730467.
Rimann, M. et al. (2016) 'Zurich university of applied sciences : center for cell biology and tissue engineering', in Roche Symposium, Basel, 2016.
Raghunath, M. et al. (2016) 'Zurich University of Applied Sciences : center for cell biology and tissue engineering', in Roche Symposium, Basel, 2016.

Mündliche Konferenzbeiträge und Abstracts

Rimann, M. et al. (2017) '3D bioprinted tissue models for substance testing', in International Conference and Exhibition - Society for Laboratory Automation and Screening (SLAS 2017), Washington, USA, 4–8 February 2017.
Rimann, M. et al. (2016) 'Bioprinted tissues for substance evaluation', in Biointerfaces International 2016 Conference, Zurich, 23-25 August 2016.

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