TEDD Partner Highlights
Biomimetic systems for cell engineering at the Institute for Bioengineering of Catalonia (IBEC)
We propose to combine engineering microfabrication technologies, tissue engineering concepts and advances in stem cell research to create cell culture microenvironments that will go beyond current 3D in vitro models.
Resulting in vitro tissue equivalents aim at recapitulating in vivo cell functionality, cell renewal and migration, multicell-type differentiation and cell-matrix and cell-cell interactions. The cell culture platforms proposed will provide physiologically relevant and highly reproducible data, and they will be compatible with conventional cell culture assays and high-throughput testing. The new organotypic cell culture platforms will aim to advance the in vitro modelling of diseases, the preclinical screening for drug toxicity, the understanding of organ development and the regenerative medicine applications.
Current main projects are:
- To engineer and validate a complex in vitro model of small intestinal mucosa.
- To engineer and validate a novel in vitro model of engineered cardiac tissue.
Visit our website at: http://www.ibecbarcelona.eu/biomsyscelleng.
Services
- Partnership to exploit applications for new models of intestinal epithelium mucosa
- Partnership to exploit applications of bioreactor chambers including perfusion and electrical stimulation capabilities
- Partnership to develop new applications for simple, low-cost, light-based biofabrication techniques
- Partnership with clinicians interested in gastrointestinal disease modelling
- Extensive expertise on surface functionalization and characterization at the micro and nanoscale
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Publications
- Castaño, AG; García-Díaz, M; Torras, N; Altay, G; Comelles, J; Martinez, E. Dynamic photopolymerization produces complex microstructures on hydrogels in a moldless approach to generate a 3D intestinal tissue model. Biofabrication 2019, 11, 025007.
- Cutrale F; Rodriguez D; Hortigüela V; Chiu C-L; Otterstrom J; Mieruszynski S; Seriola A; Larrañaga E; Raya A; Lakadamyali M; Fraser SE, Martinez E*, Ojosnegros S*. Using enhanced Number and Brightness to measure protein oligomerization dynamics in live cells, Nature Protocols 2019, 14 (2), 616-638 *shared senior authorship
- Torras, N., García-Díaz, M., Fernández-Majada, V., Martínez, E. Mimicking epithelial tissues in three-dimensional cell culture models (2018) Frontiers in Bioengineering and Biotechnology, 6, art. No. 197
- Macedo MH; Araujo G; Martinez E; Barrias C; Sarmento B. iPSC-Derived Enterocyte-like Cells for Drug Absorption and Metabolism Studies, Trends Molecular Medicine 2018, 24(8), 696-708
- Hortigüela V; Larrañaga E; Cutrale F; Seriola A; García-Díaz M; Lagunas A; Andilla J; Loza-Alvarez P; Samitier J; Ojosnegros S; Martinez E. Nanopatterns of surface-bound ephrinB1 produce multivalent ligand-receptor interactions that tune EphB2 receptor clustering Nano Letters 2018, 18, (1), 629-637.
- Ojosnegros S; Cutrale F; Rodríguez D; Otterstrom JJ; Chiu Chi-Li; Hortigüela V; Tarantino C; Seriola A; Mieruszynski S; Martínez E; Lakadamyali M; Raya A; Fraser SE. Eph-ephrin eblockng modulated by polymerization and condensation of receptors, Proceedings of the National Academy of Sciences 2017, 114, (50), 13188-13193.
- Garreta E; Oñate L; Fernández-Santos M. E; Oria R; Tarantino C; Climent A. M.; Marco A; Samitier M; Martínez E; Valls-Margarit M; Matesanz R; Taylor D. A.; Fernández-Avilés F; Izpisua Belmonte J. C.; Montserrat N. Myocardial commitment from human pluripotent stem cells: Rapid production of human heart grafts, Biomaterials 2016, 98, 64-78.
- Lagunas A, Sasso B, Tesson N, Cantos C, Martinez E, Samitier J. Synthesis of a polymethyl(methacrylate)-polystyrene-based eblock copolymer containing biotin for selective protein nanopatterning, Polymer Chemistry 2016, 7(1), 212-218.
- Lagunas, Anna, Martinez, Elena, Samitier, Josep, Surface-bound molecular gradients for the high throughput screening of cell responses, Frontiers in Bioengineering and Biotechnology 2015, 3.
- Castaño AG; Hortigüela V, Lagunas A, Cortina C; Montserrat N; Samitier J; Martínez E. Protein patterning on hydrogels by direct microcontact printing: application to cardiac differentiation, RSC Adv. 2014; 4, 29120.
- Lagunas A; Comelles J; Oberhansl S; Hortigüela V; Martínez E; Samitier J. Continuous bone morphogenetic protein-2 gradients for concentration effect studies on C2C12 osteogenic fate. Nanomedicine: Nanotechnology, Biology and Medicine. 2013; 9(5): 694 – 701.
- Comelles J; Hortigüela V; Samitier J; Martínez E. Versatile gradients of covalently bound proteins on microstructured substrates. Langmuir. 2012; 28(18): 13688 – 13697.
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Contact us
Website: www.ibecbarcelona.eu/biomsyscelleng
Location: IBEC, c/Baldiri Reixac 10-12, 08028 Barcelona, Spain