Prof. Dr. Rebecca Buller

Projects

• SINERGIA EnzyDEL / Project leader / Project ongoing
• Biocatalytic halogenation for the sustainable development of agrochemicals / Project leader / Project ongoing
• NCCR Catalysis / Team member / Project ongoing
• Strategies to reduce mycotoxins in grain side product streams enabling their reintroduction into the food value chain / Project co-leader / Project completed
• Microbial Epimerases: A Toolbox for the Synthesis of Novel Peptide-Based Drugs / Project leader / Project completed
• Impulse - Digitalization in biofabrication: Design, recombinant production and testing of innovative Protein scaffolds as inks for 3D bioprinting / Deputy project leader / Project completed
• Biocatalytic oxidation processes with alpha-ketoglutarate dependent enzymes for sustainable chemical synthesis / Project leader / Project completed
• CAS - Biocatalysis / Deputy project leader / Project completed
• Biocatalytic Production - Engineering of terpene cyclases for the production of R-alpha-Ionone / Project leader / Project completed
• PgB P-14 Engineered Halogenases / Project leader / Project completed
• Firmenich CTI 25929.2 / Project leader / Project completed
• Innovationsraum Biokatalyse P-14 / Project leader / Project completed

Publications

• Hegarty, Eimear; Büchler, Johannes; Buller, Rebecca M.,

  2023.

  Halogenases for the synthesis of small molecules.

  Current Opinion in Green and Sustainable Chemistry.

  41(100784).

  Available from: https://doi.org/10.1016/j.cogsc.2023.100784

• Papadopoulou, Athena; Meyer, Fabian; Buller, Rebecca M.,

  2022.

  Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases.

  Biochemistry.

  62(2), pp. 229-240.

  Available from: https://doi.org/10.1021/acs.biochem.2c00115

• Büchler, Johannes; Malca, Sumire Honda; Patsch, David; Voss, Moritz; Turner, Nicholas J.; Bornscheuer, Uwe T.; Allemann, Oliver; Le Chapelain, Camille; Lumbroso, Alexandre; Loiseleur, Olivier; Buller, Rebecca,

  2022.

  Algorithm-aided engineering of aliphatic halogenase WelO5* for the asymmetric late-stage functionalization of soraphens.

  Nature Communications.

  13(371).

  Available from: https://doi.org/10.1038/s41467-022-27999-1

• Büchler, Johannes; Hegarty, Eimear; Schroer, Kirsten; Snajdrova, Radka; Turner, Nicholas J.; Loiseleur, Olivier; Buller, Rebecca; Le Chapelain, Camille,

  2022.

  A collaborative journey towards the late‐stage functionalization of added‐value chemicals using engineered halogenases.

  Helvetica Chimica Acta.

  106(1), pp. e202200128.

  Available from: https://doi.org/10.1002/hlca.202200128

• Papadopoulou, Athena; Peters, Christin; Borchert, Sonja; Steiner, Kerstin; Buller, Rebecca,

  2022.

  Development of an ene reductase-based biocatalytic process for the production of flavor compounds.

  Organic Process Research & Development.

  26(7), pp. 2102-2110.

  Available from: https://doi.org/10.1021/acs.oprd.2c00096

• Voss, Moritz; Hüppi, Sean; Schaub, Daniela; Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2022.

  Enzyme engineering enables inversion of substrate stereopreference of the halogenase WelO5*.

  ChemCatChem.

  14(24), pp. e202201115.

  Available from: https://doi.org/10.1002/cctc.202201115

• Reiter, Maud; Hevey, Rachel; Buller, Rebecca; Shybeka, Inga,

  2021.

  Swiss women in chemistry – two years later ....

  Chimia.

  75(12), pp. 1088-1090.

  Available from: https://doi.org/10.2533/chimia.2021.1088

• Papadopoulou, Athena; Meierhofer, Jasmin; Meyer, Fabian; Hayashi, Takahiro; Schneider, Samuel; Sager, Emine; Buller, Rebecca,

  2021.

  Re‐programming and optimization of a L‐proline cis‐4‐hydroxylase for the cis‐3‐halogenation of its native substrate.

  ChemCatChem.

  13(18), pp. 3914-3919.

  Available from: https://doi.org/10.1002/cctc.202100591

• Meyer, Fabian; Frey, Raphael; Ligibel, Mathieu; Sager, Emine; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2021.

  Modulating chemoselectivity in a Fe(II)/α-ketoglutarate-dependent dioxygenase for the oxidative modification of a non-proteinogenic amino acid.

  ACS Catalysis.

  2021(11).

  Available from: https://doi.org/10.1021/acscatal.1c00678

• Eichenberger, Michael; Hüppi, Sean; Patsch, David; Aeberli, Natalie; Berweger, Raphael; Dossenbach, Sandro; Eichhorn, Eric; Flachsmann, Felix; Hortencio, Lucas; Voirol, Francis; Vollenweider, Sabine; Bornscheuer, Uwe T.; Buller, Rebecca,

  2021.

  Asymmetric cation-olefin monocyclization by engineered squalene-hopene cyclases.

  Angewandte Chemie: International Edition.

  60(50), pp. 26080-26086.

  Available from: https://doi.org/10.1002/anie.202108037

• Voss, Moritz; Küng, Robin; Hayashi, Takahiro; Jonczyk, Magdalena; Niklaus, Michael; Iding, Hans; Wetzl, Dennis; Buller, Rebecca,

  2020.

  Multi‐faceted set‐up of a diverse ketoreductase library enables the synthesis of pharmaceutically‐relevant secondary alcohols.

  ChemCatChem.

  13(6), pp. 1538-1545.

  Available from: https://doi.org/10.1002/cctc.202001871

• Aregger, David; Peters, Christin; Buller, Rebecca,

  2020.

  Characterization of the novel ene reductase Ppo-Er1 from paenibacillus polymyxa.

  Catalysts.

  10(2).

  Available from: https://doi.org/10.3390/catal10020254

• Voss, Moritz; Honda Malca, Sumire; Buller, Rebecca,

  2020.

  Exploring the biocatalytic potential of Fe/α‐ketoglutarate dependent halogenases.

  Chemistry - A European Journal.

  Available from: https://doi.org/10.1002/chem.201905752

• Hecht, Katrin; Meyer, Hans-Peter; Wohlgemuth, Roland; Buller, Rebecca,

  2020.

  Biocatalysis in the Swiss manufacturing environment.

  Catalysts.

  10(12), pp. 1420.

  Available from: https://doi.org/10.3390/catal10121420

• Büchler, Johannes; Papadopoulou, Athena; Buller, Rebecca,

  2019.

  Recent advances in Flavin-dependent halogenase biocatalysis : sourcing, engineering, and application.

  Catalysts.

  9(12), pp. 1030.

  Available from: https://doi.org/10.3390/catal9121030

• Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Voss, Moritz; Hunziker, Jürg; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2019.

  Evolved aliphatic halogenases enable regiocomplementary C‐H functionalization of an added‐value chemical.

  Angewandte Chemie: International Edition.

  58(51), pp. 18535-18539.

  Available from: https://doi.org/10.1002/anie.201907245

• Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Voss, Moritz; Hunziker, Jürg; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2019.

  Evolvierte aliphatische Halogenasen ermöglichen die regiokomplementäre C‐H‐Funktionalisierung einer hochwertigen Chemikalie.

  Angewandte Chemie.

  131(51), pp. 18706-18711.

  Available from: https://doi.org/10.1002/ange.201907245

• Papadopoulou, Athena; Hecht, Katrin; Buller, Rebecca,

  2019.

  Enzymatic PET degradation.

  Chimia.

  73(9), pp. 743-749.

  Available from: https://doi.org/10.2533/chimia.2019.743

• Peters, Christin; Frasson, David; Sievers, Martin; Buller, Rebecca,

  2019.

  Novel Old Yellow Enzyme subclasses.

  ChemBioChem.

  Available from: https://doi.org/10.1002/cbic.201800770

• Frey, Raphael; Hayashi, Takahiro; Buller, Rebecca,

  2019.

  Directed evolution of carbon–hydrogen bond activating enzymes.

  Current Opinion in Biotechnology.

  60, pp. 29-38.

  Available from: https://doi.org/10.1016/j.copbio.2018.12.004

• Peters, Christin; Buller, Rebecca,

  2019.

  Industrial application of 2-oxoglutarate-dependent oxygenases.

  Catalysts.

  9(3), pp. 221-240.

  Available from: https://doi.org/10.3390/catal9030221

• Peters, Christin; Buller, Rebecca,

  2019.

  Linear enzyme cascade for the production of (–)-iso-isopulegol.

  Zeitschrift für Naturforschung C.

  74(3-4).

  Available from: https://doi.org/10.1515/znc-2018-0146

• Kries, Hajo; Blomberg, Rebecca; Hilvert, Donald,

  2013.

  De novo enzymes by computational design.

  Current Opinion in Chemical Biology.

  17(2), pp. 221-228.

  Available from: https://doi.org/10.1016/j.cbpa.2013.02.012

• Blomberg, Rebecca; Kries, Hajo; Pinkas, Daniel M.; Mittl, Peer R. E.; Grütter, Markus G.; Privett, Heidi K.; Mayo, Stephen L.; Hilvert, Donald,

  2013.

  Precision is essential for efficient catalysis in an evolved Kemp eliminase.

  Nature.

  503(7476), pp. 418-421.

  Available from: https://doi.org/10.1038/nature12623

• Richter, Florian; Blomberg, Rebecca; Khare, Sagar D.; Kiss, Gert; Kuzin, Alexandre P.; Smith, Adam J. T.; Gallaher, Jasmine; Pianowski, Zbigniew; Helgeson, Roger C.; Grjasnow, Alexej; Xiao, Rong; Seetharaman, Jayaraman; Su, Min; Vorobiev, Sergey; Lew, Scott; Forouhar, Farhad; Kornhaber, Gregory J.; Hunt, John F.; Montelione, Gaetano T.; Tong, Liang; Houk, K. N.; Hilvert, Donald; Baker, David,

  2012.

  Computational design of catalytic dyads and oxyanion holes for ester hydrolysis.

  Journal of the American Chemical Society.

  134(39), pp. 16197-16206.

  Available from: https://doi.org/10.1021/ja3037367

• Privett, H. K.; Kiss, G.; Lee, T. M.; Blomberg, R.; Chica, R. A.; Thomas, L. M.; Hilvert, D.; Houk, K. N.; Mayo, S. L.,

  2012.

  Iterative approach to computational enzyme design.

  Proceedings of the National Academy of Sciences of the United States of America.

  109(10), pp. 3790-3795.

  Available from: https://doi.org/10.1073/pnas.1118082108

• Chapleau, Richard R.; Blomberg, Rebecca; Ford, Peter C.; Sagermann, Martin,

  2008.

  Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake.

  Protein Science.

  17(4), pp. 614-622.

  Available from: https://doi.org/10.1110/ps.073358908

• Hecht, Katrin; Buller, Rebecca,

  2019.

  Ene-reductases in pharmaceutical chemistry

  .

  In:

  Grunwald, Peter, ed.,

  Pharmaceutical biocatalysis : chemoenzymatic synthesis of active pharmaceutical ingredients.

  Singapore:

  Jenny Stanford.

• Buller, Rebecca; Hecht, Katrin; Mirata, Marco Antonio; Meyer, Hans-Peter,

  2017.

  An appreciation of biocatalysis in the Swiss manufacturing environment

  .

  In:

  Biocatalysis : an industrial perspective.

  Royal Society of Chemistry.

  pp. 1-43.

  Catalysis Series ; 29.

  Available from: https://doi.org/10.1039/9781782629993-00001

• Buller, Rebecca,

  2021.

  Biocatalysis and biosynthesis

  .

  In:

  Technology Outlook 2021.

  Swiss Academy of Engineering Sciences SATW.

  pp. 71.

  Available from: https://www.satw.ch/de/publikationen/technology-outlook-2021

• Buller, Rebecca; Coperét, Christophe; Emsley, Lyndon; Gademann, Karl; Hari, Yvonne; Leo, Merz,

  2021.

  Chemistry roadmap for research infrastructures 2025-2028 by the Swiss chemistry community.

  Swiss Academies Reports

  ; 16(3).

  Swiss Academy of Sciences.

  Available from: https://doi.org/10.5281/zenodo.4572642

• André, Amandine; Freimüller Leischtfeld, Susette; Mischler, Sandra; Hecht, Katrin; Stäheli, Luca; Rüegg, Ramona; Kinner, Mathias; Buller, Rebecca; Chetschik, Irene; Miescher Schwenninger, Susanne; Müller, Nadina,

  2022.

  Mycotoxins reduction strategies to reintroduce grain side product streams into the food value chain.

  In:

  20th ICC Conference, Vienna, Austria, 5-7 July 2022.

Publications before appointment at the ZHAW

Precision is essential for efficient catalysis in an evolved Kemp eliminase
Rebecca Blomberg, Hajo Kries, Daniel Pinkas, Per Mittl, Markus Grütter, Heidi Privett, Stephen Mayo, and Donald Hilvert
Nature 503, 418-421 (2013)

De novo enzymes by computational design
Hajo Kries*, Rebecca Blomberg*, and Donald Hilvert
Curr Opin Chem Biol. 17, 221-8 (2013) (* joint first authors)

Computational design of catalytic dyads and oxyanion holes for ester hydrolysis
Florian Richter*, Rebecca Blomberg*, Sagar D. Khare, Gert Kiss, Alexandre P. Kuzin, Adam J. T. Smith, Jasmine Gallaher, Zbigniew Pianowski, Roger C. Helgeson, Alexej Grjasnow, Rong Xiao, Jayaraman Seetharaman, Min Su, Sergey Vorobiev, Scott Lew, Farhad Forouhar, Gregory J. Kornhaber, John F. Hunt, Gaetano T. Montelione, Liang Tong, K. N. Houk, Donald Hilvert, and David Baker
J Am Chem Soc. 134, 16197-206 (2012) (* joint first authors)

Iterative approach to computational enzyme design
Heidi K. Privett, Gert Kiss, Toni M. Lee, Rebecca Blomberg, Roberto A. Chica, Leonard M. Thomas, Donald Hilvert, Kendall N. Houk, and Stephen L. Mayo
Proc Natl Acad Sci USA 109, 3790-5 (2012)

Design of a Highly Specific and Noninvasive Biosensor
Suitable for Real-Time in vivo Imaging of Mercury (II) Uptake
Richard Chapleau, Rebecca Blomberg, Peter Ford, and Martin Sagermann
Protein Science 17, 614-622 (2008)

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