Food Microbiology Research Group
Detection and control of food-borne pathogens
Bacteriophages are viruses that can specifically infect and thus inactivate individual bacterial species. Selected phage-isolates that are classified as ‘generally recognised as safe’ can be used as excipients in food to specifically inactivate pathogenic germs, such as Salmonella enterica or Listeria monocytogenes. Using phages has the advantage that the organoleptic properties of food are not altered. In terms of clean labelling, the use of bacteriophages can increase food safety. In our research projects we characterise new bacteriophages to control bacterial contamination in food.
Since phages can be highly host-specific, they are very well-suited to the development of new detection methods. Consequently, we are analysing phage proteins that are important for the specific adsorption of bacteria.
In challenge tests, food is deliberately contaminated with pathogenic microorganisms and/or spoilage germs in order to demonstrate the growth potential of these germs in selected products. Such tests are performed according to internationally recognised guidelines from accredited laboratories. This enables us to compare and evaluate methods for reducing the growth of bacteria. Challenge tests are also used to demonstrate the suitability of packaging for preserving food, and to investigate the effectiveness of bacteriophages or other natural substances in preventing the growth of pathogenic microorganisms. Our research includes every category of product.
We apply ISO certified procedures for the detection of microorganisms. In addition, we are researching how new detection methods for the rapid and specific recognition of microorganisms can be developed on a molecular biological basis.
Roping is a form of spoilage in unleavened bakery products containing wheat flour that is caused by contamination with Bacillus spp. As a result, breadcrumbs develop a distinct discoloration and strong volatile off-flavours. The mechanics of rope spoilage are currently not well- defined, therefore at present, only back tests can determine whether a Bacillus isolate can cause rope spoilage or not. For this reason, we are working on identifying the factors and enzymes responsible for the development of roping in order to develop a specific detection method.
Frontiers in Microbiology.
Available from : https://doi.org/10.3389/fmicb.2019.02949
Stöppelmann, Felix; Fieseler, Lars ,
van Mierlo, Joël; Hagens, Steven; Witte, Sander; Klamert, Silvia; van de Straat, Leoni; Fieseler, Lars ,
Microbiology Resource Announcements.
Available from : https://doi.org/10.1128/MRA.00078-19