Prof. Dr. Lars Fieseler

ZHAW School of Life Sciences and Facility Management

8820 Wädenswil

+41 (0) 58 934 54 07
lars.fieseler@zhaw.ch

Network

ORCID digital identifier

ORCID ID: 0000-0001-9306-6436

Projects

Zebrafish larvae for drug screening / Project leader / ongoing
Novel phage derived antimicrobials / Deputy project leader / ongoing
SONEVA / Co-project leader / completed
Contactless separation and concentration of Legionella species in drinking water installations / Project leader / completed
An effective and environmentally friendly solution to control fire blight disease caused by Erwinia amylovora in pome fruit crops / Project leader / completed
Molecular mechanism of bacteriophage tail spike proteins in bacterial infection / Co-project leader / completed
Engineered bacteriophages for the control of E. coli O157 / Project leader / completed
Persistence of STEC in grain, milling by-products, flour, and raw follow up products / Project leader / completed
Platform Health / Co-project leader / completed
Rapid Quantification of Pseudomonas aeruginosa by flow-cytometry / Project leader / completed
BIOPAC (Biopolymer based bioactive food packaging) / Team member / completed
Analysis and optimisation of a laundry process for cleaning textiles / Team member / completed

Publications

Articles in scientific journal, peer-reviewed

Dams, Dorien; Pas, Célia; Latka, Agnieszka; Drulis-Kawa, Zuzanna; Fieseler, Lars; Briers, Yves,

2025.

A VersaTile approach to reprogram the specificity of the R2-type tailocin towards different serotypes of Escherichia coli and Klebsiella pneumoniae.

Antibiotics.

14(1), pp. 104.

Available from: https://doi.org/10.3390/antibiotics14010104
Pacher, Nicola; Burtscher, Johanna; Bender, Denisse; Fieseler, Lars; Schreiner, Matthias; Domig, Konrad J.,

2024.

Operator-independent assessment of bread spoilage profiles caused by Bacillaceae reveals a high degree of inter- and intraspecies heterogeneity.

Food Research International.

197(115193).

Available from: https://doi.org/10.1016/j.foodres.2024.115193
Pas, Célia; Fieseler, Lars; Pothier, Joël F.; Briers, Yves,

2024.

Isolation, characterization, and receptor-binding protein specificity of phages PAS7, PAS59 and PAS61 infecting Shiga toxin-producing Escherichia coli O103 and O146.

Scientific Reports.

14(26050).

Available from: https://doi.org/10.1038/s41598-024-77463-x
Pacher, Nicola; Burtscher, Johanna; Bender, Denisse; Fieseler, Lars; Domig, Konrad J.,

2024.

Aerobic spore-forming bacteria associated with ropy bread : identification, characterization and spoilage potential assessment.

International Journal of Food Microbiology.

418(110730).

Available from: https://doi.org/10.1016/j.ijfoodmicro.2024.110730
Ullrich, Lisa; Gillich, Elodie; André, Amandine; Panarese, Sandra; Imhaus, Anne Flore; Fieseler, Lars; Chetschik, Irene,

2023.

Influence of ozone treatment during storage on odour-active compounds, total titratable acidity, and ascorbic acid in oranges and bananas.

Applied Sciences.

13(19), pp. 10885.

Available from: https://doi.org/10.3390/app131910885
Gayder, Steven; Kammerecker, Sandrine; Fieseler, Lars,

2023.

Biological control of the fire blight pathogen Erwinia amylovora using bacteriophages.

Journal of Plant Pathology.

106(3), pp. 853-869.

Available from: https://doi.org/10.1007/s42161-023-01478-y
Pas, Célia; Latka, Aknieszka; Fieseler, Lars; Briers, Yves,

2023.

Phage tailspike modularity and horizontal gene transfer reveals specificity towards E. coli O-antigen serogroups.

Virology Journal.

20(174).

Available from: https://doi.org/10.1186/s12985-023-02138-4
Desroche, Nicolas; De Oliveira, Déborah; Imhaus, Anne-Flore; Hupfeld, Mario; Fieseler, Lars,

2023.

Validation of N-Light™ salmonella risk test kit for detection of Salmonella spp. on environmental surfaces : AOAC Performance Tested MethodsSM 072204.

Journal of AOAC INTERNATIONAL.

106(4), pp. 939-948.

Available from: https://doi.org/10.1093/jaoacint/qsad017
Witte, Sander; Huijboom, Linda; Klamert, Silvia; van de Straat, Leoni; Hagens, Steven; Fieseler, Lars; de Vegt, Bert T.; van Mierlo, Joël T.,

2022.

Application of bacteriophages EP75 and EP335 efficiently reduces viable cell counts of Escherichia coli O157 on beef and vegetables.

Food Microbiology.

104(103978).

Available from: https://doi.org/10.1016/j.fm.2022.103978
Knecht, Leandra E.; Heinrich, Nadine; Born, Yannick; Felder, Katja; Pelludat, Cosima; Loessner, Martin J.; Fieseler, Lars; et al.,

2022.

Bacteriophage S6 requires bacterial cellulose for Erwinia amylovora infection.

Environmental Microbiology.

24(8), pp. 3436-3450.

Available from: https://doi.org/10.1111/1462-2920.15973
Pacher, Nicola; Burtscher, Johanna; Johler, Sophia; Etter, Danai; Bender, Denisse; Fieseler, Lars; Domig, Konrad J.,

2022.

Ropiness in bread : a re-emerging spoilage phenomenon.

Foods.

11(19), pp. 3021.

Available from: https://doi.org/10.3390/foods11193021
Knecht, Leandra E.; Born, Yannick; Pelludat, Cosima; Pothier, Joël F.; Smits, Theo H. M.; Loessner, Martin J.; Fieseler, Lars,

2022.

Spontaneous resistance of Erwinia amylovora against bacteriophage Y2 affects infectivity of multiple phages.

Frontiers in Microbiology.

13(908346).

Available from: https://doi.org/10.3389/fmicb.2022.908346
Larose, Delphine; Desroche, Nicolas; Reinau, Lukas; Hupfeld, Mario; Fieseler, Lars,

2021.

Validation of N-Light™ L. monocytogenes for detection of Listeria monocytogenes on environmental surfaces : AOAC Performance Tested Method℠ 122002.

Journal of AOAC INTERNATIONAL.

Available from: https://doi.org/10.1093/jaoacint/qsab165
Herren, Pascal; Fieseler, Lars; Ambuehl, Daniel; Grunder, Jürg,

2021.

Foodborne bacteria in raw drone brood of Apis mellifera – a preliminary survey.

Journal of Insects as Food and Feed.

7(3), pp. 281-285.

Available from: https://doi.org/10.3920/JIFF2020.0067
Pertics, Botond Zsombor; Szénásy, Dalma; Dunai, Dániel; Born, Yannick; Fieseler, Lars; Kovács, Tamás; Schneider, György,

2020.

Isolation of a novel lytic bacteriophage against a nosocomial methicillin-resistant Staphylococcus aureus belonging to ST45.

BioMed Research International.

2020(5463801), pp. 1-10.

Available from: https://doi.org/10.1155/2020/5463801
Ganz, Giverny; Reinau, Lukas; Imhaus, Anne Flore; Hupfeld, Mario; Fieseler, Lars,

2020.

AquaSparkTM : rapid environmental monitoring of Listeria monocytogenes.

Chimia.

74(10), pp. 791-797.

Available from: https://doi.org/10.2533/chimia.2020.791
Knecht, Leandra E.; Veljkovic, Marjan; Fieseler, Lars,

2020.

Diversity and function of phage encoded depolymerases.

Frontiers in Microbiology.

10(2949).

Available from: https://doi.org/10.3389/fmicb.2019.02949
van Mierlo, Joël; Hagens, Steven; Witte, Sander; Klamert, Silvia; van de Straat, Leoni; Fieseler, Lars,

2019.

Complete genome sequences of escherichia coli phages vB_EcoM-EP75 and vB_EcoP-EP335.

Microbiology Resource Announcements.

8(16).

Available from: https://doi.org/10.1128/MRA.00078-19
Born, Yannick; Knecht, Leandra E.; Eigenmann, Mirjam; Bolliger, Michel; Klumpp, Jochen; Fieseler, Lars,

2019.

A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types.

Archives of Virology.

164(3), pp. 819-830.

Available from: https://doi.org/10.1007/s00705-019-04148-6
Roth-Konforti, Michal; Green, Ori; Hupfeld, Mario; Fieseler, Lars; Heinrich, Nadine; Ihssen, Julian; Vorberg, Raffael; Wick, Lukas; Spitz, Urs; Shabat, Doron,

2019.

Ultrasensitive detection of salmonella and listeria monocytogenes by small-molecule chemiluminescence probes.

Angewandte Chemie: International Edition.

58(30), pp. 10361-10367.

Available from: https://doi.org/10.1002/anie.201904719
Knecht, Leandra E.; Born, Yannick; Pothier, Joël F.; Loessner, Martin J.; Fieseler, Lars,

2018.

Complete genome sequences of erwinia amylovora phages vB_EamP-S2 and vB_EamM-Bue1.

Microbiology Resource Announcements.

7(3).

Available from: https://doi.org/10.1128/MRA.00891-18
Buttimer, Colin; Born, Yannick; Lucid, Alan; Loessner, Martin J.; Fieseler, Lars; Coffey, Aidan,

2018.

Erwinia amylovora phage vB_EamM_Y3 represents another lineage of hairy myoviridae.

Research in Microbiology.

169(9), pp. 505-514.

Available from: https://doi.org/10.1016/j.resmic.2018.04.006
Born, Yannick; Fieseler, Lars; Thöny, Valentin; Leimer, Nadja; Duffy, Brion; Loessner, Martin J.,

2017.

Engineering of bacteriophages Y2::dpoL1-C and Y2::luxAB for efficient control and rapid detection of the fire blight pathogen, Erwinia amylovora.

Applied and Environmental Microbiology.

83(12), pp. e00341-17.

Available from: https://doi.org/10.1128/AEM.00341-17
Shen, Yang; Boulos, Samy; Sumrall, Eric; Gerber, Benjamin; Julian-Rodero, Alicia; Eugster, Marcel R.; Fieseler, Lars; Nyström, Laura; Ebert, Marc-Olivier; Loessner, Martin J.,

2017.

Structural and functional diversity inListeriacell wall teichoic acids.

Journal of Biological Chemistry.

292(43), pp. 17832-17844.

Available from: https://doi.org/10.1074/jbc.M117.813964
Schmitter, Sibylle; Fieseler, Lars; Klumpp, Jochen; Bertram, Ralph; Loessner, Martin J.,

2017.

TetR-dependent gene regulation in intracellular Listeria monocytogenes demonstrates the spatiotemporal surface distribution of ActA.

Molecular Microbiology.

105(3), pp. 413-425.

Available from: https://doi.org/10.1111/mmi.13706
Krapf, Tamara; Kuhn, Roger; Kauf, Peter; Gantenbein-Demarchi, Corinne; Fieseler, Lars,

2016.

Quantitative real time PCR does not reliably detect single fecal indicator bacteria in drinking water.

Water Science and Technology.

16(6), pp. 1674-1682.

Available from: https://doi.org/10.2166/ws.2016.052
Schwendimann, Livia Maya; Kauf, Peter; Fieseler, Lars; Gantenbein-Demarchi, Corinne; Miescher Schwenninger, Susanne,

2015.

Development of a quantitative PCR assay for rapid detection of Lactobacillus plantarum and Lactobacillus fermentum in cocoa bean fermentation.

Journal of Microbiological Methods.

115, pp. 94-99.

Available from: https://doi.org/10.1016/j.mimet.2015.05.022
Born, Yannick; Bosshard, Lars; Duffy, Brion; Loessner, Martin; Fieseler, Lars,

2015.

Protection of Erwinia amylovora bacteriophage Y2 from UV-induced damage by natural compounds.

Bacteriophage.

5(4), pp. e1074330.

Available from: https://doi.org/10.1080/21597081.2015.1074330
Fieseler, Lars; Doyscher, Dominik; Loessner, Martin; Schuppler, Markus,

2014.

Acanthamoeba release compounds which promote growth of Listeria monocytogenes and other bacteria.

Applied Microbiology and Biotechnology.

98(7), pp. 3091-3097.

Available from: https://doi.org/10.1007/s00253-014-5534-9
Born, Yannick; Fieseler, Lars; Klumpp, Jochen; Eugster, Marcel R.; Zurfluh, Katrin; Duffy, Brion; Loessner, Martin J.,

2014.

The tail-associated depolymerase of Erwinia amylovoraphage L1 mediates host cell adsorption and enzymatic capsule removal, which can enhance infection by other phage.

Environmental Microbiology.

16(7), pp. 2168-2180.

Available from: https://doi.org/10.1111/1462-2920.12212
Doyscher, Dominik; Fieseler, Lars; Dons, Lone; Loessner, Martin J.; Schuppler, Markus,

2013.

Acanthamoeba feature a unique backpacking strategy to trap and feed on Listeria monocytogenes and other motile bacteria.

Environmental Microbiology.

15(2), pp. 433-446.

Available from: https://doi.org/10.1111/j.1462-2920.2012.02858.x
Guenther, Susanne; Herzig, Oliver; Fieseler, Lars; Klumpp, Jochen; Loessner, Martin J.,

2012.

Biocontrol of Salmonella Typhimurium in RTE foods with the virulent bacteriophage FO1-E2.

International Journal of Food Microbiology.

154(1-2), pp. 66-72.

Available from: https://doi.org/10.1016/j.ijfoodmicro.2011.12.023
Born, Yannick; Fieseler, Lars; Marazzi, Janine; Lurz, Rudi; Duffy, Brion; Loessner, Martin J.,

2011.

Novel virulent and broad-host-range Erwinia amylovora bacteriophages reveal a high degree of mosaicism and a relationship to enterobacteriaceae phages.

Applied and Environmental Microbiology.

77(17), pp. 5945-5954.

Available from: https://doi.org/10.1128/AEM.03022-10

Book parts, peer-reviewed

Fieseler, Lars; Loessner, Martin,

2013.

Bacteriophage biocontrol.

In:

Labbé, Ronald G.; García, Santos, eds.,

Guide to foodborne pathogens.

New York:

Wiley.

pp. 448-456.

Available from: https://doi.org/10.1002/9781118684856.ch28

Conference contributions, peer-reviewed

Gayder, Steven; Fieseler, Lars; Castle, Alan; Svircev, Antonet,

2022.

A novel genome-wide, qPCR-based approach to identify genetic determinants of phage host range[poster].

In:

Viruses of Microbes (VoM), Guimarães, Portugal, 18-22 July 2022.

ZHAW Zürcher Hochschule für Angewandte Wissenschaften.

Available from: https://doi.org/10.21256/zhaw-27325
Knecht, Leandra; Heinrich, Nadine; Felder, Katja; Born, Yannick; Pelludat, Cosima; Loessner, Martin; Fieseler, Lars,

2018.

Mutations in the bcs operon of Erwinia amylovora abolish infection by bacteriophages S6 and M7[poster].

In:

Viruses of Microbes (VoM), Wroclaw, Poland, 9-13 July 2018.
Buttimer, Collin; Born, Yannick; Hendrix, Hanne; Oliveria, Hugo; McAuliffe, Olivia; Neve, Horst; Ross, R. Paul; Hill, Colin; Noben, Jean-Paul; O'Mahony, Jim; Fieseler, Lars; Lavigne, Rob; Coffey, Aidan; et al.,

2017.

Hairy jumbo phages of Enterobacteriaceae[poster].

In:

2017 Evergreen International Phage Meeting, Evergreen State College, USA, 6-11 August 2017.

ResearchGate.

Available from: https://doi.org/10.13140/RG.2.2.19768.42243

Other publications

Gerber, Nicole; Fieseler, Lars; Hirsch, Sven; Neutsch, Lukas; Papadopoulou, Athina; Smits, Theo,

2022.

Zusammenarbeit und wissenschaftlicher Austausch sind der Schlüssel zu erfolgreicher Forschung : der ZHAW Health Research Hub bringt’s unter einen Hut.

Clinicum.

2022(6), pp. 80-81.

Available from: https://doi.org/10.21256/zhaw-26376
Stöppelmann, Felix; Fieseler, Lars,

2020.

Massnahmen zur Beherrschung mikrobiologischer Risiken verursacht durch Vibrio spp..
Wetzel, Silvan; Fieseler, Lars,

2020.

Risk evaluation of E. coli ST 131 as a foodborne pathogen in Switzerland.
Wetzel, Silvan; Fieseler, Lars,

2020.

Shiga-toxin producing escherichia coli in flour, wheat grain and flour products.
Knecht, Leandra; Born, Yannick; Loessner, Martin; Fieseler, Lars,

2016.

Identification of phage receptors in Erwinia amylovora after transposon mutagenesis[poster].

In:

Viruses of Microbes (VoM), Liverpool, United Kingdom, 18-22 July 2016.
Freimüller Leischtfeld, Susette; Weber, Stefan; Kuhn, Roger; Schmid, Jürg; Fieseler, Lars,

2015.

Molekulare Schnellmethode für den Legionellen-Test.

Alimenta.

2015(8).
Born, Yannick; Fieseler, Lars,

2015.

A bacteriophage cocktail to control Salmonella Typhimurium[poster].

In:

21st Biennial Evergreen International Phage Biology Meeting, Olympia, USA, 2-7 August 2015.
Born, Yannick; Knecht, Leandra; Pelludat, Cosima; Duffy, Brion; Loessner, Martin; Fieseler, Lars,

2015.

Bakteriophagen zur Bekämpfung von Feuerbrand[poster].

In:

1. Nationale Tagung - Gemeinsam gegen Feuerbrand, Wädenswil, 2015.
Adlhart, Christian; Born, Yannick; Fuchs, Michael; Fieseler, Lars; Pavlovic, Branislav,

2015.

Encapsulation and controlled release of bacteriophages for food protection using electrospun nanofibers[paper].

In:

Proceedings of the NART conference 2015.

NART 2015, Nanofibers, Applications and Related Technologies, Liberec, Czech Republic, 31 August - 2 September 2015.

pp. 49-56.
Gantenbein-Demarchi, Corinne; Kuhn, Roger; Kauf, Peter; Schmid, Jürg; Krapf, Tamara; Fieseler, Lars,

2015.

Fäkalindikatoren im Trinkwasser nachweisen.

Alimenta.
Born, Yannick; Klumpp, Jochen; Zurfluh, Katrin; Duffy, Brion; Loessner, Martin; Fieseler, Lars,

2015.

Implications of the phage depolymerase DpoL1 on Erwinia amylovora[poster].

In:

21st Biennial Evergreen International Phage Biology Meeting, Olympia, USA, 2-7 August 2015.
Leiblein, Thomas; Kroslakova, Ivana; Freimüller Leischtfeld, Susette; Fieseler, Lars; Hofer, Susanne,

2015.

Legionella in FM : Detection of Legionella pneumophila in waterlines of dental surgeries in the Canton of Zurich, Switzerland[paper].

In:

Alexander, Keith; Price, Ilfryn, eds.,

EuroFM Research Papers, Section 5, Development Papers.

EuroFM Conference 2015, Glasgow, Ireland, 1-3 June 2015.

European Facilities Management Network (EuroFM).

pp. 26-33.
Fieseler, Lars,

2015.

Nachweis und Kontrolle der Keimbelastung im Lebensmittel.

Lebensmittel-Technologie.

2015(4), pp. 12-13.
Fieseler, Lars; Gantenbein-Demarchi, Corinne; Miescher Schwenninger, Susanne; Kuhn, Roger; Kleinert, Michael,

2013.

Rope in bread : a critical challenge for the bakery industry[poster].

In:

4th MoniQA International Conference, Budapest, Hungary, 26 February - 1 March 2013.
Gantenbein-Demarchi, Corinne; Kuhn, Roger; Krapf, Tamara; Fieseler, Lars,

2012.

Detection of faecal indicator organisms by RT-qPCR[poster].

In:

SGM Annual Congress 2012 - Swiss Society for Microbiology, St. Gallen, 21-22 June 2012.
Fieseler, Lars; Loessner, Martin; Hagens, Steven,

2011.

Bacteriophages and food safety.

In:

Lacroix, Christophe, ed.,

Protective cultures, antimicrobial metabolites and bacteriophages for food and beverage biopreservation.

Oxford:

Woodhead Publishing.

pp. 161-178.

Woodhead Publishing Series in Food Science, Technology and Nutrition ; 201.

Available from: https://doi.org/10.1533/9780857090522.1.161
Heller, Knut; Loessner, Martin; Fieseler, Lars,

2011.

Bakteriophagen : Grundlagen, Rolle in Lebensmitteln und Anwendungen zum Nachweis sowie der Kontrolle von Krankheitserregern.

1. Auflage.

Hamburg:

Behr.

ISBN 978-3-89947-594-4.

Oral conference contributions and abstracts

Gayder, Steven; Kammerecker, Sandrine; Gervásio, Kellen; Henriques, André; Torres-Cortés, Gloria; Kőrösiné Papp, Szilvia; Kovács, Tamás; Raffai, Krisztina; Cabezón Largas, Sixto; de Santos Prieto, Borja; Fieseler, Lars,

2022.

A qPCR-based, population dynamics approach for the development of a bacteriophage-based biopesticide.

In:

Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.
Kammerecker, Sandrine; Gayder, Steven; Gervásio, Kellen; Henriques, André; Torres-Cortés, Gloria; Kőrösiné Papp, Szilvia; Kovács, Tamás; Raffai, Krisztina; Cabezón Largas, Sixto; de Santos Prieto, Borja; Fieseler, Lars,

2022.

PhageFire : formulation of a bacteriophage-based biopesticide against Erwinia amylovora.

In:

Third International Symposium on Fire Blight of Rosaceous Plants, Dresden-Pillnitz, Germany, 6-9 September 2022.
Fieseler, Lars,

2017.

Bacteriophages for the control of pathogenic bacteria.

In:

BioTech 2017 Conference, Prague, Czech Republic, 13-17 June 2017.
Born, Yannick; Leimer, Nadja; Thöny, Valentin; Zurfluh, Katrin; Eugster, Marcel; Klumpp, Jochen; Duffy, Brion; Loessner, Martin; Fieseler, Lars,

2016.

Engineered phages for efficient control and rapid detection of viable Erwinia amylovora cells.

In:

Viruses of Microbes (VoM), Liverpool, United Kingdom, 18-22 July 2016.
Born, Yannick; Fieseler, Lars,

2016.

The effectiveness of T5-like phages to control Salmonella enterica.

In:

3rd International Conference «Bacteriophages», Moscow, Russia, 13-15 October 2016.
Fieseler, Lars; Klumpp, Jochen,

2014.

A cocktail of two bacteriophages is required to control Salmonella in vitro.

In:

Viruses of Microbes (VoM), Zurich, Switzerland, 14-18 July 2014.
Fieseler, Lars,

2014.

Bakteriophagen zur Kontrolle von Salmonella Typhimurium in Lebensmitteln.

In:

7. Symposium: Schnellmethoden und Automatisierung in der Lebensmittel‐Mikrobiologie, Lemgo, Germany, 1-2 July 2014.
Hinderling, Christian; Kroslakova, Ivana; Gantenbein-Demarchi, Corinne; Freimüller Leischtfeld, Susette; Delueg, Stephanie; Miescher Schwenninger, Susanne; Fieseler, Lars,

2012.

Development of an efficient strategy for the identification of the dominant microflora of cocoa fermentation.

In:

1st Swiss International Congress on Industrial Microbiology (MICROSCON), Olten, Switzerland, 16-17 October 2012.

go back