XhortOMICs: Diagnostic and epidemiological tools for the Xanthomonas hortorum species-level clade based on OMICs technologies

At a glance

Project leader : Dr. Theo Smits
Co-project leader : Dr. Joël Pothier
Project team : Nay Dia
Project budget : CHF 273'576
Project status : completed
Funding partner : SNSF (COST / Projekt Nr. 177064)
Contact person : Theo Smits

Description

Despite its recent re-emergence and increased prevalence in many countries, the research on Xanthomonas hortorum species-level clade members has been very limited until now. It is thus important to have an increased knowledge thereof to be able to react to its increasing occurrence. Therefore, the overall aim of the SNF funded project called XhortOMICs is to study the genomic diversity of the different pathovars within the X. hortorum species-level clade in order to design novel diagnostic methods and to study the molecular base of host-specificity of the individual pathovars. Genomic data of X. hortorum species-level clade members are largely unavailable, and this project aims at filling that substantial gap. By establishing the genome sequences of representative isolates from this clade, this project will also fill in an important information gap with insights on population ecology, virulence, genetics and evolution. This will also allow designing genomics informed assays for rapid, easy and in-field diagnostics in order to contribute to a prevention of further spreading/introduction of these pathogens. Additionally, this project will study host-specificity and pathogenicity factors by setting up plant assays for bacterial mutant phenotyping.

Further information

https://euroxanth.eu/

Publications

Costa, Joana; Pothier, Joël F.; Bosis, Eran; Boch, Jens; Kölliker, Roland; Koebnik, Ralf,

2023.

A community-curated DokuWiki resource on diagnostics, diversity, pathogenicity and genetic control of Xanthomonads.

Molecular Plant-Microbe Interactions.

Available from: https://doi.org/10.1094/MPMI-11-23-0184-FI
Costa, Joana; Pothier, Joël F.; Boch, Jens; Stefani, Emilio; Koebnik, Ralf,

2022.

Integrating science on Xanthomonas and Xylella for integrated plant disease management.

Microorganisms.

11(1), pp. 6.

Available from: https://doi.org/10.3390/microorganisms11010006
Dia, Nay C.; Cottyn, Bart; Blom, Jochen; Smits, Theo H. M.; Pothier, Joël F.,

2022.

Differentiation of the Xanthomonas hortorum – Xanthomonas hydrangeae species complex using sensitive and selective LAMP assays.

Frontiers in Agronomy.

4(898778).

Available from: https://doi.org/10.3389/fagro.2022.898778
Dia, Nay C.; Morinière, Lucas; Cottyn, Bart; Bernal, Eduardo; Jacobs, Jonathan M.; Koebnik, Ralf; Osdaghi, Ebrahim; Potnis, Neha; Pothier, Joël F.,

2022.

Xanthomonas hortorum – beyond gardens : current taxonomy, genomics, and virulence repertoires.

Molecular Plant Pathology.

23(5), pp. 597-621.

Available from: https://doi.org/10.1111/mpp.13185
Hakalová, Eliška; Čechová, Jana; Tekielska, Dorota A.; Eichmeier, Ales; Pothier, Joël F.,

2022.

Combined effect of thyme and clove phenolic compounds on Xanthomonas campestris pv. campestris and biocontrol of black rot disease on cabbage seeds.

Frontiers in Microbiology.

13.

Available from: https://doi.org/10.3389/fmicb.2022.1007988
Dia, Nay C.; Van Vaerenbergh, Johan; Van Malderghem, Cinzia; Blom, Jochen; Smits, Theo H. M.; Cottyn, Bart; Pothier, Joël F.,

2021.

Xanthomonas hydrangeae sp. nov., a novel plant pathogen isolated from Hydrangea arborescens.

International Journal of Systematic and Evolutionary Microbiology.

71(12).

Available from: https://doi.org/10.1099/ijsem.0.005163
Costa, Joana; Pothier, Joël F.; Boch, Jens; Stefani, Emilio; Jacques, Marie-Agnès; Catara, Vittoria; Koebnik, Ralf,

2021.

Integrating science on Xanthomonadaceae for sustainable plant disease management in Europe.

Molecular Plant Pathology.

22(12), pp. 1461-1463.

Available from: https://doi.org/10.1111/mpp.13150
Cottyn, B.; De Paepe, B.; Dia, Nay; Van Malderghem, C.; Pothier, Joël; Van Vaerenbergh, J.,

2021.

First report of bacterial leaf spot of Hydrangea in retail nurseries in Belgium caused by strains assigned to a new Xanthomonas hortorum clade.

New Disease Reports.

43(2), pp. e12008.

Available from: https://doi.org/10.1002/ndr2.12008
Catara, Vittoria; Cubero, Jaime; Pothier, Joël; Bosis, Eran; Bragard, Claude; Đermić, Edyta; Holeva, Maria C.; Jacques, Marie-Agnès; Petter, Francoise; Pruvost, Olivier; Robène, Isabelle; Studholme, David J.; Tavares, Fernando; Vicente, Joana G.; Koebnik, Ralf; Costa, Joana,

2021.

Trends in molecular diagnosis and diversity studies for phytosanitary regulated Xanthomonas.

Microorganisms.

9(4), pp. 862.

Available from: https://doi.org/10.3390/microorganisms9040862
Fernandes, Camila; Martins, Leonor; Teixeira, Miguel; Blom, Jochen; Pothier, Joël; Fonseca, Nuno A.; Tavares, Fernando,

2021.

Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree.

Microorganisms.

9(3), pp. 624.

Available from: https://doi.org/10.3390/microorganisms9030624
Dia, Nay C.; Rezzonico, Fabio; Smits, Theo H. M.; Pothier, Joël F.,

2020.

Complete or high-quality draft genome sequences of six Xanthomonas hortorum strains sequenced with short- and long-read technologies.

Microbiology Resource Announcements.

9(41), pp. e00828-20.

Available from: https://doi.org/10.1128/MRA.00828-20
Martins, Leonor; Fernandes, Camila; Blom, Jochen; Dia, Nay C.; Pothier, Joël F.; Tavares, Fernando,

2020.

Xanthomonas euroxanthea sp. nov. : a new xanthomonad species including pathogenic and non-pathogenic strains of walnut.

International Journal of Systematic and Evolutionary Microbiology.

70(12), pp. 6024-6031.

Available from: https://doi.org/10.1099/ijsem.0.004386

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