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Effect of cryopreservation on genome mutation rate - examples of grapevine

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Grapevine genetic resources are maintained in field collections being challenged by repeating biotic and abiotic stresses. Plant adapt to stress by increased mutation rates. Hence, long-term field collections may not be optimal to maintain original cultivars (and clones in grapevine). This project will quantify cryopreservation process-induced genome mutation rate in grapevine by next generation sequencing .