Comparative genomics of the plant pathogen Xanthomonas arboricola pv. pruni and related xanthomonads

At a glance

Project leader : Dr. Joël Pothier
Project team : Michael Gétaz, Dr. Theo Smits
Project status : ongoing
Funding partner : Internal
Project partner : Justus-Liebig-Universität Giessen / Bioinformatics and Systems Biology
Contact person : Joël Pothier

Description

Xanthomonas arboricola pv. pruni (Xap) causes bacterial spot and canker on a wide range of Prunus spp. (e.g., apricot, peach, plum, al-mond, cherry). Outbreaks on stone fruits can result in severe economic losses to fruit quality and yield, branch/tree dieback, and orchard devastation. Xap is endemic in the USA, NZ and is a quarantine regulated pathogen in Europe and elsewhere. Control options for this pathogen are actually quite limited and there is an increasing invasion risk with 1) climate change and 2) wide host range of this pathogen.

Despite the regulatory and economic significance of Xap, surprisingly little is known about its genetics. Genetic studies have for the most part been limited to biodiversity analysis. We apply genomics in order to identify this invasive pathogen Achilles’ heel (e.g., virulence or fitness determinants) that can be exploited for development of improved control compounds and phytosanitary strategies. We have sequenced the complete genome of a genotypic-representative Xap strain from Europe, use our assembled genome as a template for assembly of sequences of other pathovars and are pursuing comparative genomics with other Xanthomonas species that have been sequenced.

Publications

Kałużna, Monika; Prokić, Andjelka; Obradović, Aleksa; Weldon, William A.; Stockwell, Virginia O.; Pothier, Joël F.,

2023.

Specific and sensitive detection tools for Xanthomonas arboricola pv. corylina, the causal agent of bacterial blight of hazelnut, developed with comparative genomics.

Frontiers in Plant Science.

14(1254107).

Available from: https://doi.org/10.3389/fpls.2023.1254107
Silva, Kayla Gisela; Martins, Leonor; Teixeira, Miguel; Pothier, Joël F.; Tavares, Fernando,

2022.

DNA markers for detection and genotyping of Xanthomonas euroxanthea.

Microorganisms.

10(6), pp. 1078.

Available from: https://doi.org/10.3390/microorganisms10061078
Kałużna, Monika; Pothier, Joël F.,

2022.

Complete genome sequence data of two Xanthomonas arboricola strains isolated from blueberry plants displaying bacterial leaf blight in Poland.

Phytopathology.

112(8), pp. 1814-1818.

Available from: https://doi.org/10.1094/PHYTO-11-21-0484-A
Kałużna, Monika; Fischer‐Le Saux, Marion; Pothier, Joël; Jacques, Marie‐Agnès; Obradović, Aleksa; Tavares, Fernando; Stefani, Emilio,

2021.

Xanthomonas arboricola pv. juglandis and pv. corylina: Brothers or distant relatives? : genetic clues, epidemiology, and insights for disease management.

Molecular Plant Pathology.

22(12), pp. 1481-1499.

Available from: https://doi.org/10.1111/mpp.13073
Fernandes, Camila; Martins, Leonor; Teixeira, Miguel; Blom, Jochen; Pothier, Joël; Fonseca, Nuno A.; Tavares, Fernando,

2021.

Comparative genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis strains isolated from a single walnut host tree.

Microorganisms.

9(3), pp. 624.

Available from: https://doi.org/10.3390/microorganisms9030624
Pothier, Joël F.; Kałużna, Monika; Prokić, Andjelka; Obradovic, Aleksa; Rezzonico, Fabio,

2021.

Complete genome and plasmid sequence data of three Xanthomonas arboricola pv. corylina strains, the bacterium responsible for bacterial blight of hazelnut.

Phytopathology.

112(4), pp. 956-960.

Available from: https://doi.org/10.1094/PHYTO-08-21-0356-A
Fernandes, Camila; Blom, Jochen; Pothier, Joël F.; Tavares, Fernando,

2018.

High-quality draft genome sequence of xanthomonas arboricola pv. juglandis CPBF 1521, isolated from leaves of a symptomatic walnut tree in Portugal without a past of phytosanitary treatment.

Microbiology Resource Announcements.

7(16).

Available from: https://doi.org/10.1128/MRA.00887-18
Fernandes, Camila; Blom, Jochen; Pothier, Joël F.; Tavares, Fernando,

2018.

High-Quality draft genome sequence of xanthomonas sp. strain CPBF 424, a walnut-pathogenic strain with atypical features.

Microbiology Resource Announcements.

7(15).

Available from: https://doi.org/10.1128/MRA.00921-18
Palacio-Bielsa, Ana; López-Soriano, Pablo; Bühlmann, Andreas; van Doorn, Joop; Pham, Khanh; Cambra, Miguel A.; Berruete, Isabel M.; Pothier, Joël F.; Duffy, Brion; Olmos, Antonio; López, María M.,

2015.

Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of Xanthomonas arboricola pv. pruni in plant tissue samples.

Journal of Microbiological Methods.

112, pp. 36-39.

Available from: https://doi.org/10.1016/j.mimet.2015.03.005
Vandroemme, Joachim; Cottyn, Bart; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Maes, Martine,

2013.

Xanthomonas arboricola pv. fragariae : what's in a name?.

Plant Pathology.

62(5), pp. 1123-1131.

Available from: https://doi.org/10.1111/ppa.12028
Socquet-Juglard, Didier; Duffy, Brion; Pothier, Joël F.; Christen, Danilo; Gessler, Cesare; Pattochi, Andrea,

2013.

Identification of a major QTL for Xanthomonas arboricola pv. pruni resistance in apricot.

Tree Genetics & Genomes.

9(2), pp. 409-421.

Available from: https://doi.org/10.1007/s11295-012-0562-z
Socquet-Juglard, Didier; Kamber, Tim; Pothier, Joël F.; Christen, Danilo; Gessler, Cesare; Duffy, Brion; Patocchi, Andrea,

2013.

Comparative RNA-Seq analysis of early-infected peach leaves by the invasive phytopathogen Xanthomonas arboricola pv. pruni.

PLOS ONE.

8(1).

Available from: https://doi.org/10.1371/journal.pone.0054196
Bühlmann, Andreas; Pothier, Joël F.; Tomlinson, Jennifer A.; Frey, Jürg F.; Boonham, N.; Smits, Theo H.M.; Duffy, Brion,

2012.

Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenic Xanthomonas arboricola pv. pruni at the intraspecific level.

Plant Pathology.

62(2), pp. 475-484.

Available from: https://doi.org/10.1111/j.1365-3059.2012.02654.x

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