Publications
Articles
2024
GEMS: A Generalizable GNN Framework For Protein-Ligand Binding Affinity Prediction Through Robust Data Filtering and Language Model Integration. D. Graber, P. Stockinger, Fabian Meyer, Siddhartha Mishra, Claus Horn, & R. Buller. BioRxiv December 11, 2024. https://biorxiv.org/cgi/content/short/2024.12.09.627482v1
Structure Prediction and Computational Protein Design for Efficient Biocatalysts and Bioactive Proteins. R. Buller, J. Damborsky, D. Hilvert & U. T. Bornscheuer. Angewandte Chemie (2024) November 25. https://doi.org/10.1002/anie.202421686
Nature’s Toolbox for the Hydrolysis of Lactams and Cyclic Imides. P. Stockinger & R. M. Buller. ACS Catalysis (2024) October 16. https://doi.org/10.1021/acscatal.4c04474
Enriching productive mutational paths accelerates enzyme evolution. D. Patsch, T. Schwander, M. Voss, D. Schaub, S. Hüppi, M. Eichenberger, P. Stockinger, L. Schelbert, S. Giger, F. Peccati, G. Jiménez-Osés, M. Mutný, A. Krause, U. T. Bornscheuer, D. Hilvert & R. M. Buller. Nature Chemical Biology (2024) September 11 https://doi.org/10.1038/s41589-024-01712-3
A new physical and biological strategy to reduce the content of zearalenone in infected wheat kernels: the effect of cold needle perforation, microorganisms, and purified enzyme. A. André, K. Hecht, S. Mischler, L Stäheli, F. Kerhanaj, R. Buller, M. Kinner, S. Freimüller Leischtfeld, I. Chetschik, S. Miescher Schwenninger & N. Müller. Food Research International (2024) June 6. https://doi.org/10.1016/j.foodres.2024.114364.
Elucidating the Role of O2 Uncoupling for the Adaptation of Bacterial Biodegradation Reactions Catalyzed by Rieske Oxygenases. C. E. Bopp, N. M. Bernet, F. Meyer, R. Khan, S. L. Robinson, H.-P. E. Kohler, R. Buller & T. B. Hofstetter. ACS ENVIRONMENTAL AU (2024) May 25. https://doi.org/10.1021/acsenvironau.4c00016.
Excelzyme: A Swiss University−Industry Collaboration for Accelerated Biocatalyst Development. S. H. Malca, P. Stockinger, N. Duss, Daniela Milbredt, H. Iding & R. Buller. CHIMIA (2024) March 27. https://doi:10.2533/chimia.2024.108.
Effective engineering of a ketoreductase for the biocatalytic synthesis of an ipatasertib precursor. S. Honda Malca, N. Duss, J. Meierhofer, D. Patsch, M. Niklaus, S. Reiter, S. P. Hanlon, D. Wetzl, B. Kuhn, H. Iding & R. Buller. Communications Chemistry (2024) February 28. https://doi.org/10.1038/s42004-024-01130-5
2023
From nature to industry: Harnessing enzymes for biocatalysis. R. Buller, S. Lutz, R. J. Kazlauskas, R. Snajdrova, J. C. Moore & U. T. Bornscheuer. Science (2023) November 24. https://doi.org/10.1126/science.adh8615.
LibGENiE – A bioinformatic pipeline for the design of information-enriched enzyme libraries. D. Patsch, M. Eichenberger, M. Voss, U. T. Bornscheuer & R. M. Buller. Computational and Structural Biotechnology Journal 14 September 2023. https://doi.org/10.1016/j.csbj.2023.09.013.
The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis. M. Eichenberger, T. Schwander, S. Hüppi, J. Kreuzer, P. R. E. Mittl, F. Peccati, G. Jiménez-Osés, M. Naesby & R. M. Buller. Nature Catalysis August 31, 2023. doi.org/10.1038/s41929-023-01018-y.
Advances in Noncanonical Amino Acid Incorporation for Enzyme Engineering Applications. S. Giger & R. Buller. Chimia June 28th, 2023. doi.org/10.2533/chimia.2023.395.
Improving Enzyme Fitness with Machine Learning. D. Patsch & R. Buller. Chimia March 29th, 2023. https://doi.org/10.2533/chimia.2023.116
Halogenases for the synthesis of small molecules. E. Hegarty, J. Büchler & R. Buller. Current Opinion in Green and Sustainable Chemistry, February 10, 2023. https://doi.org/10.1016/j.cogsc.2023.100784
2022
A Collaborative Journey towards the Late‐Stage Functionalization of Added‐Value Chemicals Using Engineered Halogenases. J. Büchler, E. Hegarty, K. Schroer, R. Snajdrova, N. J. Turner, O. Loiseleur, R. Buller & C. Le Chapelain. Helvetica Chimica Acta November 2022. https://10.1002/hlca.202200128
Enzyme engineering enables inversion of substrate stereopreference of the halogenase WelO5*. M. Voss, S. Hüppi, D. Schaub, T. Hayashi, M. Ligibel, E. Sager, K. Schroer, R. Snajdrova & R. M. U. Buller. ChemCatChem October 24, 2022. https://doi.org/10.1002/cctc.202201115
Development of an Ene Reductase-Based Biocatalytic Process for the Production of Flavor Compounds. A. Papadopoulou, C. Peters, S. Borchert, K. Steiner & R. Buller. Org. Process Res. Dev. June 14, 2022. https://pubs.acs.org/doi/10.1021/acs.oprd.2c00096
Engineering Fe(III)/α-Ketoglutarate-Dependent Halogenases and Desaturases. A. Papadopoulou, F. Meyer & R. Buller. Biochemistry April 21, 2022. https://doi.org/10.1021/acs.biochem.2c00115
Algorithm-aided engineering of aliphatic halogenase WelO5* for the asymmetric late-stage functionalization of soraphens. J. Büchler, S. Honda Malca, D. Patsch, M. Voss, N. J. Turner, U. T. Bornscheuer, O. Allemann, C. Le Chapelain, A. Lumbroso, O. Loiseleur & R. Buller. Nature Communications. January 18, 2022. https://doi.org/10.1038/s41467-022-27999-1
2021
Asymmetric Cation-Olefin Monocyclization by Engineered Squalene-Hopene Cyclases. M. Eichenberger, S. Hüppi, D. Patsch, N. Aeberli, R. Berweger, S. Dossenbach, E. Eichhorn, F. Flachsmann, L. Hortencio, F. Voirol, S. Vollenweider, U. Bornscheuer, R. M. U. Buller. Angesandte Chemie. August 4, 2021. https://doi.org/10.1002/anie.202108037
“Re-programming and optimization of a L-proline cis-4-hydroxylase for the cis-3-halogenation of its native substrate“ A. Papadopoulou, J. Meierhofer, F. Meyer, T. Hayashi, S. Schneider, E. Sager, R. M. U. Buller. ChemCatChem. June 7, 2021.https://doi.org/10.1002/cctc.202100591
“Modulating Chemoselectivity in a Fe(II)/α-Ketoglutarate-Dependent Dioxygenase for the Oxidative Modification of a Nonproteinogenic Amino Acid” F. Meyer, R. Frey, M.Ligibel, E. Sager, K. Schroer, R. Snajdrova, R. Buller. ACS Catalysis. May 10, 2021. https://doi.org/10.1021/acscatal.1c00678
2020
“Multi‐faceted set‐up of a diverse ketoreductase library enables the synthesis of pharmaceutically‐relevant secondary alcohols” M. Voss, R. Küng, T. Hayashi, M. Jonczyk, M. Niklaus, H. Iding, D. Wetzl, R. M. U. Buller. ChemCatChem. December 11, 2020. https://doi.org/10.1002/cctc.202001871
“Biocatalysis in the Swiss Manufacturing Environment” K. Hecht, H.-P. Meyer, R. Wohlgemuth, R. Buller. Catalysts. December 4, 2020. https://doi.org/10.3390/catal10121420
“Characterization of the novel Ene Reductase Ppo-Er1 from Paenibacillus polymyxa” D. Aregger, C. Peters, R. M. Buller. Catalysts. February 19, 2020. doi.org/10.3390/catal10020254.
“Exploring the biocatalytic potential of Fe/α-ketoglutarate dependent halogenases” M. Voss, S. H. Malca, R. Buller. Chemistry - A European Journal. January 22, 2020. doi.org/10.1002/chem.201905752.
2019
“Recent Advances in Flavin-Dependent Halogenase Biocatalysis: Sourcing, Engineering, and Application” J. Büchler, A. Papadopoulou, R. Buller. Catalysts. December 5, 2019. https://doi.org/10.3390/catal9121030.
“Evolved Aliphatic Halogenases Enable Regiocomplementary C-H Functionalization of an Added-Value Chemical” T. Hayashi, M. Ligibel, E. Sager, M. Voss, J. Hunziker, K. Schroer, R. Snajdrova, R. Buller. Angewandte Chemie. October 7, 2019. doi.org/10.1002/anie.201907245.
“Enzymatic PET Degradation” A. Papadopoulou, K. Hecht, R. Buller. CHIMIA. September 2019. doi.org/10.2533/chimia.2019.743.
“Industrial Application of 2-Oxoglutarate-Dependent Oxgenases” C. Peters, R. M. Buller. Catalysts February 28, 2019. doi.org/10.3390/catal9030221.
“Novel Old Yellow Enzyme Subclasses” C. Peters, D. Frasson, M. Sievers, R. Buller. ChemBioChem. Feb. 2019. 13 February 13, 2019. doi.org/10.1002/cbic.201800770
“Linear enzyme cascade for the production of (-)-iso-isopulegol” C. Peters, R. Buller. Zeitung für Naturforschung C. January 11, 2019. doi.org/10.1515/znc-2018-0146
2018
“Directed evolution of carbon-hydrogen bond activating enzymes” R. Frey, T. Hayashi, R. M. Buller. Current Opinion in Biotechnology. December 21, 2018. doi.org/10.1016/j.copbio.2018.12.004
Bookchapters
“Ene-Reductases in Pharmaceutical Chemistry” K. Hecht, R. Buller. Pharmaceutical Biocatalysis: Chemoenzymatic Synthesis of Active Pharmaceutical Ingredients December 2019.
“An Appreciation of Biocatalysis in the Swiss Manufacturing Environment” R. Buller, K. Hecht, M. A. Mirata and H-P. Meyer. Biocatalysis: An Industrial Perspective. November 9, 2017
Videos
Others
"Biocatalytic Potential of alpha alpha-Ketoglutarate Dependent Dioxygenases (PDF 529 kB)" J. Taubitz ÁJour September 2021. (in German)
"Engineering of Epimerases towards Pharmaceutical Applications (PDF 472 kB)" R. Giger. ÁJour September 2021. (in German)
“Biokatalyse und Biosynthese - Nachhaltige chemische Produktion” R. Buller. Technology Outlook – SATW. April 2019.
“10th Wädenswil Day of Life Sciences Hosts 2nd CCBIO Symposium Industrial Biocatalysis”. K. Hecht. Chimia. September 2018. doi.org/10.2533/chimia.2018.661.