Prof. Dr. Rebecca Buller

Arbeit an der ZHAW

Tätigkeit an der ZHAW

Head of Competence Center for Biocatalysis
Head of Center for Biocatalysis, Environmental and Process Technology
Lecturer Biochemical Engineering

Aus- und Weiterbildung

Arbeits- und Forschungsschwerpunkte, Spezialkenntnisse

Biocatalysis
Biotransformation
Process Development
Process Scale-Up
Enzyme Engineering
Directed Evolution

Beruflicher Werdegang

Since 10/2015
Head of Competence Center for Biocatalysis
Lecturer for Biochemical Engineering
ZHAW - University of Applied Sciences, Wädenswil, Switzerland

10/2014 - 09/2015
Senior Scientist, Laboratory Head, Firmenich SA, Geneva, Switzerland
Development of Biocatalytic Processes

05/2011 - 09/2014
Scientist, Laboratory Head, Firmenich SA, Geneva, Switzerland
Development of Biocatalytic Processes

Aus- und Fortbildung

11/2006 – 02/2011
Ph.D. studies, Institute of Organic Chemistry
Department of Chemistry and Applied Biosciences
ETH Zurich, Switzerland
Supervisor Prof. Dr. Donald Hilvert
“Directed Evolution of Artificial Enzymes”

09/2006
Chemistry Diploma, Institute of Biochemistry
Westfälische Wilhelms Universität, Münster, Germany
Supervisor Prof. Dr. Hans-Joachim Galla
“Expression and functional characterization of an ABC- transporter in vitro"

11/2004 – 11/2005
Visiting Researcher, Department of Chemistry and Biochemistry
University of California, Santa Barbara, USA
Supervisors Prof. Dr. Peter Ford and Prof. Dr. Martin Sagermann
“S-Nitrosation of proteins: Investigation of functional and structural modifications”
“Engineering of a biological NO sensor”

10/2001 – 09/2006
Undergradutae and graduate studies of Chemistry
Westfälische Wilhelms Universität, Münster, Germany

Projekte

• SINERGIA EnzyDEL / ProjektleiterIn / Projekt laufend
• Biocatalytic halogenation for the sustainable development of agrochemicals / ProjektleiterIn / Projekt laufend
• NCCR Catalysis / Teammitglied / Projekt laufend
• Strategien zur Reduktion von Mykotoxinen in Getreidenebenproduktströmen / Co-ProjektleiterIn / Projekt abgeschlossen
• Microbial Epimerases: A Toolbox for the Synthesis of Novel Peptide-Based Drugs / ProjektleiterIn / Projekt abgeschlossen
• Impulse - Digitalization in biofabrication: Design, recombinant production and testing of innovative Protein scaffolds as inks for 3D bioprinting / Stellv. ProjektleiterIn / Projekt abgeschlossen
• Biocatalytic oxidation processes with alpha-ketoglutarate dependent enzymes for sustainable chemical synthesis / ProjektleiterIn / Projekt abgeschlossen
• CAS - Biocatalysis / Stellv. ProjektleiterIn / Projekt abgeschlossen
• Biocatalytic Production - Engineering of terpene cyclases for the production of R-alpha-Ionone / ProjektleiterIn / Projekt abgeschlossen
• PgB P-14 Engineered Halogenases / ProjektleiterIn / Projekt abgeschlossen
• Firmenich CTI 25929.2 / ProjektleiterIn / Projekt abgeschlossen
• Innovationsraum Biokatalyse P-14 / ProjektleiterIn / Projekt abgeschlossen

Publikationen

• Hegarty, Eimear; Büchler, Johannes; Buller, Rebecca M.,

  2023.

  Halogenases for the synthesis of small molecules.

  Current Opinion in Green and Sustainable Chemistry.

  41(100784).

  Verfügbar unter: https://doi.org/10.1016/j.cogsc.2023.100784

• Papadopoulou, Athena; Meyer, Fabian; Buller, Rebecca M.,

  2022.

  Engineering Fe(II)/α-ketoglutarate-dependent halogenases and desaturases.

  Biochemistry.

  62(2), S. 229-240.

  Verfügbar unter: https://doi.org/10.1021/acs.biochem.2c00115

• Büchler, Johannes; Malca, Sumire Honda; Patsch, David; Voss, Moritz; Turner, Nicholas J.; Bornscheuer, Uwe T.; Allemann, Oliver; Le Chapelain, Camille; Lumbroso, Alexandre; Loiseleur, Olivier; Buller, Rebecca,

  2022.

  Algorithm-aided engineering of aliphatic halogenase WelO5* for the asymmetric late-stage functionalization of soraphens.

  Nature Communications.

  13(371).

  Verfügbar unter: https://doi.org/10.1038/s41467-022-27999-1

• Büchler, Johannes; Hegarty, Eimear; Schroer, Kirsten; Snajdrova, Radka; Turner, Nicholas J.; Loiseleur, Olivier; Buller, Rebecca; Le Chapelain, Camille,

  2022.

  A collaborative journey towards the late‐stage functionalization of added‐value chemicals using engineered halogenases.

  Helvetica Chimica Acta.

  106(1), S. e202200128.

  Verfügbar unter: https://doi.org/10.1002/hlca.202200128

• Papadopoulou, Athena; Peters, Christin; Borchert, Sonja; Steiner, Kerstin; Buller, Rebecca,

  2022.

  Development of an ene reductase-based biocatalytic process for the production of flavor compounds.

  Organic Process Research & Development.

  26(7), S. 2102-2110.

  Verfügbar unter: https://doi.org/10.1021/acs.oprd.2c00096

• Voss, Moritz; Hüppi, Sean; Schaub, Daniela; Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2022.

  Enzyme engineering enables inversion of substrate stereopreference of the halogenase WelO5*.

  ChemCatChem.

  14(24), S. e202201115.

  Verfügbar unter: https://doi.org/10.1002/cctc.202201115

• Reiter, Maud; Hevey, Rachel; Buller, Rebecca; Shybeka, Inga,

  2021.

  Swiss women in chemistry – two years later ....

  Chimia.

  75(12), S. 1088-1090.

  Verfügbar unter: https://doi.org/10.2533/chimia.2021.1088

• Papadopoulou, Athena; Meierhofer, Jasmin; Meyer, Fabian; Hayashi, Takahiro; Schneider, Samuel; Sager, Emine; Buller, Rebecca,

  2021.

  Re‐programming and optimization of a L‐proline cis‐4‐hydroxylase for the cis‐3‐halogenation of its native substrate.

  ChemCatChem.

  13(18), S. 3914-3919.

  Verfügbar unter: https://doi.org/10.1002/cctc.202100591

• Meyer, Fabian; Frey, Raphael; Ligibel, Mathieu; Sager, Emine; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2021.

  Modulating chemoselectivity in a Fe(II)/α-ketoglutarate-dependent dioxygenase for the oxidative modification of a non-proteinogenic amino acid.

  ACS Catalysis.

  2021(11).

  Verfügbar unter: https://doi.org/10.1021/acscatal.1c00678

• Eichenberger, Michael; Hüppi, Sean; Patsch, David; Aeberli, Natalie; Berweger, Raphael; Dossenbach, Sandro; Eichhorn, Eric; Flachsmann, Felix; Hortencio, Lucas; Voirol, Francis; Vollenweider, Sabine; Bornscheuer, Uwe T.; Buller, Rebecca,

  2021.

  Asymmetric cation-olefin monocyclization by engineered squalene-hopene cyclases.

  Angewandte Chemie: International Edition.

  60(50), S. 26080-26086.

  Verfügbar unter: https://doi.org/10.1002/anie.202108037

• Voss, Moritz; Küng, Robin; Hayashi, Takahiro; Jonczyk, Magdalena; Niklaus, Michael; Iding, Hans; Wetzl, Dennis; Buller, Rebecca,

  2020.

  Multi‐faceted set‐up of a diverse ketoreductase library enables the synthesis of pharmaceutically‐relevant secondary alcohols.

  ChemCatChem.

  13(6), S. 1538-1545.

  Verfügbar unter: https://doi.org/10.1002/cctc.202001871

• Aregger, David; Peters, Christin; Buller, Rebecca,

  2020.

  Characterization of the novel ene reductase Ppo-Er1 from paenibacillus polymyxa.

  Catalysts.

  10(2).

  Verfügbar unter: https://doi.org/10.3390/catal10020254

• Voss, Moritz; Honda Malca, Sumire; Buller, Rebecca,

  2020.

  Exploring the biocatalytic potential of Fe/α‐ketoglutarate dependent halogenases.

  Chemistry - A European Journal.

  Verfügbar unter: https://doi.org/10.1002/chem.201905752

• Hecht, Katrin; Meyer, Hans-Peter; Wohlgemuth, Roland; Buller, Rebecca,

  2020.

  Biocatalysis in the Swiss manufacturing environment.

  Catalysts.

  10(12), S. 1420.

  Verfügbar unter: https://doi.org/10.3390/catal10121420

• Büchler, Johannes; Papadopoulou, Athena; Buller, Rebecca,

  2019.

  Recent advances in Flavin-dependent halogenase biocatalysis : sourcing, engineering, and application.

  Catalysts.

  9(12), S. 1030.

  Verfügbar unter: https://doi.org/10.3390/catal9121030

• Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Voss, Moritz; Hunziker, Jürg; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2019.

  Evolved aliphatic halogenases enable regiocomplementary C‐H functionalization of an added‐value chemical.

  Angewandte Chemie: International Edition.

  58(51), S. 18535-18539.

  Verfügbar unter: https://doi.org/10.1002/anie.201907245

• Hayashi, Takahiro; Ligibel, Mathieu; Sager, Emine; Voss, Moritz; Hunziker, Jürg; Schroer, Kirsten; Snajdrova, Radka; Buller, Rebecca,

  2019.

  Evolvierte aliphatische Halogenasen ermöglichen die regiokomplementäre C‐H‐Funktionalisierung einer hochwertigen Chemikalie.

  Angewandte Chemie.

  131(51), S. 18706-18711.

  Verfügbar unter: https://doi.org/10.1002/ange.201907245

• Papadopoulou, Athena; Hecht, Katrin; Buller, Rebecca,

  2019.

  Enzymatic PET degradation.

  Chimia.

  73(9), S. 743-749.

  Verfügbar unter: https://doi.org/10.2533/chimia.2019.743

• Peters, Christin; Frasson, David; Sievers, Martin; Buller, Rebecca,

  2019.

  Novel Old Yellow Enzyme subclasses.

  ChemBioChem.

  Verfügbar unter: https://doi.org/10.1002/cbic.201800770

• Frey, Raphael; Hayashi, Takahiro; Buller, Rebecca,

  2019.

  Directed evolution of carbon–hydrogen bond activating enzymes.

  Current Opinion in Biotechnology.

  60, S. 29-38.

  Verfügbar unter: https://doi.org/10.1016/j.copbio.2018.12.004

• Peters, Christin; Buller, Rebecca,

  2019.

  Industrial application of 2-oxoglutarate-dependent oxygenases.

  Catalysts.

  9(3), S. 221-240.

  Verfügbar unter: https://doi.org/10.3390/catal9030221

• Peters, Christin; Buller, Rebecca,

  2019.

  Linear enzyme cascade for the production of (–)-iso-isopulegol.

  Zeitschrift für Naturforschung C.

  74(3-4).

  Verfügbar unter: https://doi.org/10.1515/znc-2018-0146

• Kries, Hajo; Blomberg, Rebecca; Hilvert, Donald,

  2013.

  De novo enzymes by computational design.

  Current Opinion in Chemical Biology.

  17(2), S. 221-228.

  Verfügbar unter: https://doi.org/10.1016/j.cbpa.2013.02.012

• Blomberg, Rebecca; Kries, Hajo; Pinkas, Daniel M.; Mittl, Peer R. E.; Grütter, Markus G.; Privett, Heidi K.; Mayo, Stephen L.; Hilvert, Donald,

  2013.

  Precision is essential for efficient catalysis in an evolved Kemp eliminase.

  Nature.

  503(7476), S. 418-421.

  Verfügbar unter: https://doi.org/10.1038/nature12623

• Richter, Florian; Blomberg, Rebecca; Khare, Sagar D.; Kiss, Gert; Kuzin, Alexandre P.; Smith, Adam J. T.; Gallaher, Jasmine; Pianowski, Zbigniew; Helgeson, Roger C.; Grjasnow, Alexej; Xiao, Rong; Seetharaman, Jayaraman; Su, Min; Vorobiev, Sergey; Lew, Scott; Forouhar, Farhad; Kornhaber, Gregory J.; Hunt, John F.; Montelione, Gaetano T.; Tong, Liang; Houk, K. N.; Hilvert, Donald; Baker, David,

  2012.

  Computational design of catalytic dyads and oxyanion holes for ester hydrolysis.

  Journal of the American Chemical Society.

  134(39), S. 16197-16206.

  Verfügbar unter: https://doi.org/10.1021/ja3037367

• Privett, H. K.; Kiss, G.; Lee, T. M.; Blomberg, R.; Chica, R. A.; Thomas, L. M.; Hilvert, D.; Houk, K. N.; Mayo, S. L.,

  2012.

  Iterative approach to computational enzyme design.

  Proceedings of the National Academy of Sciences of the United States of America.

  109(10), S. 3790-3795.

  Verfügbar unter: https://doi.org/10.1073/pnas.1118082108

• Chapleau, Richard R.; Blomberg, Rebecca; Ford, Peter C.; Sagermann, Martin,

  2008.

  Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake.

  Protein Science.

  17(4), S. 614-622.

  Verfügbar unter: https://doi.org/10.1110/ps.073358908

• Hecht, Katrin; Buller, Rebecca,

  2019.

  Ene-reductases in pharmaceutical chemistry

  .

  In:

  Grunwald, Peter, Hrsg.,

  Pharmaceutical biocatalysis : chemoenzymatic synthesis of active pharmaceutical ingredients.

  Singapore:

  Jenny Stanford.

• Buller, Rebecca; Hecht, Katrin; Mirata, Marco Antonio; Meyer, Hans-Peter,

  2017.

  An appreciation of biocatalysis in the Swiss manufacturing environment

  .

  In:

  Biocatalysis : an industrial perspective.

  Royal Society of Chemistry.

  S. 1-43.

  Catalysis Series ; 29.

  Verfügbar unter: https://doi.org/10.1039/9781782629993-00001

• Buller, Rebecca,

  2021.

  Biocatalysis and biosynthesis

  .

  In:

  Technology Outlook 2021.

  Swiss Academy of Engineering Sciences SATW.

  S. 71.

  Verfügbar unter: https://www.satw.ch/de/publikationen/technology-outlook-2021

• Buller, Rebecca; Coperét, Christophe; Emsley, Lyndon; Gademann, Karl; Hari, Yvonne; Leo, Merz,

  2021.

  Chemistry roadmap for research infrastructures 2025-2028 by the Swiss chemistry community.

  Swiss Academies Reports

  ; 16(3).

  Swiss Academy of Sciences.

  Verfügbar unter: https://doi.org/10.5281/zenodo.4572642

• André, Amandine; Freimüller Leischtfeld, Susette; Mischler, Sandra; Hecht, Katrin; Stäheli, Luca; Rüegg, Ramona; Kinner, Mathias; Buller, Rebecca; Chetschik, Irene; Miescher Schwenninger, Susanne; Müller, Nadina,

  2022.

  Mycotoxins reduction strategies to reintroduce grain side product streams into the food value chain.

  In:

  20th ICC Conference, Vienna, Austria, 5-7 July 2022.

Publikationen vor Tätigkeit an der ZHAW

Precision is essential for efficient catalysis in an evolved Kemp eliminase
Rebecca Blomberg, Hajo Kries, Daniel Pinkas, Per Mittl, Markus Grütter, Heidi Privett, Stephen Mayo, and Donald Hilvert
Nature 503, 418-421 (2013)

De novo enzymes by computational design
Hajo Kries*, Rebecca Blomberg*, and Donald Hilvert
Curr Opin Chem Biol. 17, 221-8 (2013) (* joint first authors)

Computational design of catalytic dyads and oxyanion holes for ester hydrolysis
Florian Richter*, Rebecca Blomberg*, Sagar D. Khare, Gert Kiss, Alexandre P. Kuzin, Adam J. T. Smith, Jasmine Gallaher, Zbigniew Pianowski, Roger C. Helgeson, Alexej Grjasnow, Rong Xiao, Jayaraman Seetharaman, Min Su, Sergey Vorobiev, Scott Lew, Farhad Forouhar, Gregory J. Kornhaber, John F. Hunt, Gaetano T. Montelione, Liang Tong, K. N. Houk, Donald Hilvert, and David Baker
J Am Chem Soc. 134, 16197-206 (2012) (* joint first authors)

Iterative approach to computational enzyme design
Heidi K. Privett, Gert Kiss, Toni M. Lee, Rebecca Blomberg, Roberto A. Chica, Leonard M. Thomas, Donald Hilvert, Kendall N. Houk, and Stephen L. Mayo
Proc Natl Acad Sci USA 109, 3790-5 (2012)

Design of a Highly Specific and Noninvasive Biosensor
Suitable for Real-Time in vivo Imaging of Mercury (II) Uptake
Richard Chapleau, Rebecca Blomberg, Peter Ford, and Martin Sagermann
Protein Science 17, 614-622 (2008)

Zurück