Spatial mRNA Sequencing on DNBSEQ-G400

Peripheral glial cells drive early wound healing; however, while repair glial cells are known to secrete tissue remodeling factors, their roles in late-stage tissue repair and fibrosis remain poorly understood.

Using state-of-the-art omics approaches, in particular Stereo-seq, we will define the transcriptional states of repair glia and characterize their intercellular communication within the late-stage wound microenvironment. Stereo-seq, commercialized by STOmics, leverages high-density DNA nanoball arrays to achieve near single-cell spatial resolution, enabling comprehensive mapping of gene expression directly in intact tissue sections. By comparing control and glia-depleted wounds, we will identify cell populations that interact with repair glia, define ligand–receptor signaling pairs, and determine how glial cells shape the spatial organization of remodeling skin tissue.

This approach will allow us to resolve glial cell states at high resolution and reconstruct their interaction networks, with a particular focus on communication between repair glial cells, fibroblasts, and immune cells. Together, these analyses will reveal how repair glia contribute to late-stage tissue remodeling and fibrosis.