Prof. Dr. Jack Rohrer

Projects

• A platform enabling efficient and effective delivery and packaging of therapeutic molecules / Project leader / completed
• Novel cell-based immunization technology and functional validation of resulting antibodies / Project leader / completed
• Antibody discovery for therapy of pain / Project leader / completed

Publications

Articles in scientific journal, peer-reviewed

• Beffinger, M., Schellhammer, L., Taşkoparan, B., Deplazes, S., Salazar, U., Tatari, N., Seehusen, F., von Balthazar, L., Zinner, C., Spath, S., Shekarian, T., Ritz, M.-F., McDaid, M., Egloff, P., Zimmermann, I., Okada, H., Ward, E. S., Rohrer, J., Seeger, M. A., et al. (2025). FcRn-silencing of IL-12Fc prevents toxicity of local IL-12 therapy and prolongs survival in experimental glioblastoma. Nature Communications, 2025(16), 4751. https://doi.org/10.1038/s41467-025-59971-0
• Sabani, B., Brand, M., Albert, I., Inderbitzin, J., Eichenseher, F., Schmelcher, M., Rohrer, J., Riedl, R., & Lehmann, S. (2023). A novel surface functionalization platform to prime extracellular vesicles for targeted therapy and diagnostic imaging. Nanomedicine: Nanotechnology, Biology and Medicine, 47(102607). https://doi.org/10.1016/j.nano.2022.102607
• Kozono, T., Sato, H., Okumura, W., Jogano, C., Tamura-Nakano, M., Kawamura, Y. I., Rohrer, J., Tonozuka, T., & Nishikawa, A. (2021). The N-terminal region of Jaw1 has a role to inhibit the formation of organized smooth endoplasmic reticulum as an intrinsically disordered region. Scientific Reports, 11(753). https://doi.org/10.1038/s41598-020-80258-5
• Luginbühl, V., Meier, N., Kovar, K., & Rohrer, J. (2018). Intracellular drug delivery : potential usefulness of engineered Shiga toxin subunit B for targeted cancer therapy. Biotechnology Advances, 36(3), 613–623. https://doi.org/10.1016/j.biotechadv.2018.02.005
• Peanne, R., Legrand, D., Duvet, S., Mir, A.-M., Matthijs, G., Rohrer, J., & Foulquier, F. (2011). Differential effects of lobe A and lobe B of the conserved oligomeric golgi complex on the stability of β1,4-galactosyltransferase 1 and α2,6-sialyltransferase 1. Glycobiology, 21(7), 864–876. https://doi.org/10.1093/glycob/cwq176
• Schaub, B. E., Berger, E. G., & Rohrer, J. (2008). Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cellular and Molecular Life Sciences, 65(22), 3677–3687. https://doi.org/10.1007/s00018-008-8446-y
• Schaub, B. E., Berger, B., Berger, E. G., & Rohrer, J. (2006). Transition of galactosyltransferase 1 from trans-golgi cisterna to the trans-golgi network is signal mediated. Molecular Biology of the Cell, 17(12), 5153–5162. https://doi.org/10.1091/mbc.e06-08-0665
• Nair, P., Schaub, B. E., Huang, K., Chen, X., Murphy, R. F., Griffith, J. M., Geuze, H. J., & Rohrer, J. (2005). Characterization of the TGN exit signal of the human mannose 6-phosphate uncovering enzyme. Journal of Cell Science, 118(13), 2949–2956. https://doi.org/10.1242/jcs.02434
• Pulipparacharuvil, S., Akbar, M., Ray, S., Sevrioukov, E. A., Haberman, A. S., Rohrer, J., & Krämer, H. (2005). Drosophila Vps16A is required for trafficking to lysosomes and biogenesis of pigment granules. Journal of Cell Science, 118(16), 3663–3673. https://doi.org/10.1242/jcs.02502
• Stöckli, J., & Rohrer, J. (2004). The palmitoyltransferase of the cation-dependent mannose 6-phosphate receptor cycles between the plasma membrane and endosomes. Molecular Biology of the Cell, 15(6), 2617–2626. https://doi.org/10.1091/mbc.e03-11-0808
• Müntener, K., Zwicky, R., Csucs, G., Rohrer, J., & Baici, A. (2004). Exon skipping of cathepsin B : mitochondrial targeting of a lysosomal peptidase provokes cell death. Journal of Biological Chemistry, 279(39), 41012–41017. https://doi.org/10.1074/jbc.M405333200
• Stöckli, J., Höning, S., & Rohrer, J. (2004). The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding. Journal of Biological Chemistry, 279(22), 23542–23549. https://doi.org/10.1074/jbc.M313525200
• Nair, P., Schaub, B. E., & Rohrer, J. (2003). Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor. Journal of Biological Chemistry, 278(27), 24753–24758. https://doi.org/10.1074/jbc.M300174200
• Berger, E. G., & Rohrer, J. (2003). Galactosyltransferase — still up and running. Biochimie, 85(3-4), 261–274. https://doi.org/10.1016/S0300-9084(03)00008-7
• Crottet, P., Meyer, D. M., Rohrer, J., & Spiess, M. (2002). ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes. Molecular Biology of the Cell, 13(10), 3672–3682. https://doi.org/10.1091/mbc.e02-05-0309
• Mathews, P. M., Guerra, C. B., Jiang, Y., Grbovic, O. M., Kao, B. H., Schmidt, S. D., Dinakar, R., Mercken, M., Hille-Rehfeld, A., Rohrer, J., Mehta, P., Cataldo, A. M., & Nixon, R. A. (2002). Alzheimer’s disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Aβ secretion : role for altered lysosomal hydrolase distribution in β-amyloidogenesis. Journal of Biological Chemistry, 277(7), 5299–5307. https://doi.org/10.1074/jbc.M108161200
• Lee, W.-S., Rohrer, J., Kornfeld, R., & Kornfeld, S. (2002). Multiple signals regulate trafficking of the mannose 6-phosphate-uncovering enzyme. Journal of Biological Chemistry, 277(5), 3544–3551. https://doi.org/10.1074/jbc.M108531200
• Rohrer, J., & Kornfeld, R. (2001). Lysosomal hydrolase mannose 6-phosphate uncovering enzyme resides in the trans-Golgi network. Molecular Biology of the Cell, 12(6), 1623–1631. https://doi.org/10.1091/mbc.12.6.1623
• Schweizer, A., Stahl, P. D., & Rohrer, J. (2000). A di-aromatic motif in the cytosolic tail of the mannose receptor mediates endosomal sorting. Journal of Biological Chemistry, 275(38), 29694–29700. https://doi.org/10.1074/jbc.M000571200
• Schweizer, A., Valdenaire, O., Köster, A., Lang, Y., Schmitt, G., Lenz, B., Bluethmann, H., & Rohrer, J. (1999). Neonatal lethality in mice deficient in XCE, a novel member of the endothelin-converting enzyme and neutral endopeptidase family. Journal of Biological Chemistry, 274(29), 20450–20456. https://doi.org/10.1074/jbc.274.29.20450
• Schweizer, A., Löffler, B.-M., & Rohrer, J. (1999). Palmitoylation of the three isoforms of human endothelin-converting enzyme-1. Biochemical Journal, 340(3), 649–656. https://doi.org/10.1042/bj3400649
• Olayioye, M. A., Graus-Porta, D., Beerli, R. R., Rohrer, J., Gay, B., & Hynes, N. E. (1998). ErbB-1 and ErbB-2 acquire distinct signaling properties dependent upon their dimerization partner. Molecular and Cellular Biology, 18(9), 5042–5051. https://doi.org/10.1128/MCB.18.9.5042
• Schweizer, A., Kornfeld, S., & Rohrer, J. (1997). Proper sorting of the cation-dependent mannose 6-phosphate receptor in endosomes depends on a pair of aromatic amino acids in its cytoplasmic tail. Proceedings of the National Academy of Sciences of the United States of America, 94(26), 14471–14476. https://doi.org/10.1073/pnas.94.26.14471
• Hicke, L., Zanolari, B., Pypaert, M., Rohrer, J., & Riezman, H. (1997). Transport through the yeast endocytic pathway occurs through morphologically distinct compartments and requires an active secretory pathway and Sec18p/N-ethylmaleimide-sensitive fusion protein. Molecular Biology of the Cell, 8(1), 13–31. https://doi.org/10.1091/mbc.8.1.13
• Schweizer, A., Kornfeld, S., & Rohrer, J. (1996). Cysteine34 of the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor is reversibly palmitoylated and required for normal trafficking and lysosomal enzyme sorting. Journal of Cell Biology, 132(4), 577–584. https://doi.org/10.1083/jcb.132.4.577
• Rohrer, J., Schweizer, A., Russel, D., & Kornfeld, S. (1996). The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. Journal of Cell Biology, 132(4), 565–576. https://doi.org/10.1083/jcb.132.4.565
• Schweizer, A., Rohrer, J., Slot, J. W., Geuze, H. J., & Kornfeld, S. (1995). Reassessment of the subcellular localization of p63. Journal of Cell Science, 108, 2477–2485. http://jcs.biologists.org/content/108/6/2477
• Rohrer, J., Schweizer, A., Johnson, K. F., & Kornfeld, S. (1995). A determinant in the cytoplasmic tail of the cation-dependent mannose 6-phosphate receptor prevents trafficking to lysosomes. Journal of Cell Biology, 130(6), 1297–1306. https://doi.org/10.1083/jcb.130.6.1297
• Schweizer, A., Rohrer, J., & Kornfeld, S. (1995). Determination of the structural requirements for palmitoylation of p63. Journal of Biological Chemistry, 270(16), 9638–9644. https://doi.org/10.1074/jbc.270.16.9638
• Schweizer, A., Rohrer, J., Hauri, H., & Kornfeld, S. (1994). Retention of p63 in an ER-Golgi intermediate compartment depends on the presence of all three of its domains and on its ability to form oligomers. Journal of Cell Biology, 126(1), 25–39. https://doi.org/10.1083/jcb.126.1.25
• Schweizer, A., Rohrer, J., Jenö, P., DeMaio, A., Buchman, T. G., & Hauri, H.-P. (1993). A reversibly palmitoylated resident protein (p63) of an ER-golgi intermediate compartment is related to a circulatory shock resuscitation protein. Journal of Cell Science, 104, 685–694. http://jcs.biologists.org/content/104/3/685.short
• Rohrer, J., Bénédetti, H., Zanolari, B., & Riezman, H. (1993). Identification of a novel sequence mediating regulated endocytosis of the G protein-coupled alpha-pheromone receptor in yeast. Molecular Biology of the Cell, 4(5), 511–521. https://doi.org/10.1091/mbc.4.5.511
• Raths, S., Rohrer, J., Crausaz, F., & Riezman, H. (1993). end3 and end4 : two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. Journal of Cell Biology, 120(1), 55–65. https://doi.org/10.1083/jcb.120.1.55
• Rohrer, J., & Kuhn, A. (1990). The function of a leader peptide in translocating charged amino acyl residues across a membrane. Science, 250(4986), 1418–1421. https://doi.org/10.1126/science.2124001
• Kuhn, A., Rohrer, J., & Gallusser, A. (1990). Bacteriophages M13 and Pf3 tell us how proteins insert into the membrane. Journal of Structural Biology, 104(1-3), 38–43. https://doi.org/10.1016/1047-8477(90)90055-H

Other publications

• Rimann, M., Mathes, S., Raghunath, M., & Rohrer, J. (2016). Zurich university of applied sciences : center for cell biology and tissue engineering. Roche Symposium, Basel, 2016.
• Raghunath, M., Rimann, M., Mathes, S., & Rohrer, J. (2016). Zurich University of Applied Sciences : center for cell biology and tissue engineering. Roche Symposium, Basel, 2016.
• Schaub, B. E., Nair, P., & Rohrer, J. (2005). Analysis of protein transport to lysosomes. Current Protocols in Cell Biology, 27(1). https://doi.org/10.1002/0471143030.cb1508s27

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