Dr. Evelyn Wolfram

Projects

• GACP in Cannabis cultivation / Deputy project leader / completed
• Computational literature-based natural product drug discovery / Team member / completed
• Biopolymer based bioactive food packaging / Team member / completed

Publications

Articles in scientific journal, peer-reviewed

• Hofer, N., Wolfram, E. and Urmann, C. (2026) 'Shedding light on an active principle from the aerial parts of Echinacea purpurea against SARS-CoV-2 in vitro', Phytochemistry Letters, 74(104179). doi: 10.1016/j.phytol.2026.104179.
• Peter, S. et al. (2026) 'Inter-laboratory comparison of photometric and LC assay methods for hydroxyanthracene glycosides determination in senna herbal drugs and dry extracts', Planta Medica. doi: 10.1055/a-2828-2181.
• Vahekeni, N. et al. (2026) 'Historical texts as a potential resource for plant-based antiviral agents against SARS-CoV-2 : the example of the Receptarium of Burkhard III von Hallwyl from 16th-century Switzerland', Frontiers in Pharmacology, 16(1731629). doi: 10.3389/fphar.2025.1731629.
• Jalil, B. et al. (2025) ''Walking the EQUATOR : from the CONSORT statement (1996) to better reporting in the 'science of botanicals'/ethnopharmacology', Journal of Ethnopharmacology, 358(120901). doi: 10.1016/j.jep.2025.120901.
• Patwardhan, B. et al. (2025) 'A global approach to safety assessment of medicinal plants', Bulletin of the World Health Organization, 103(11), pp. 741–743. doi: 10.2471/blt.24.292879.
• Hook, I. et al. (2025) 'Factors influencing clinical trials of herbal medicinal products – using ginger as example', Planta Medica, 91(15), pp. 880–890. doi: 10.1055/a-2686-6315.
• Trendafilova, A. et al. (2024) 'Unfolding phenolics biosynthetic plasticity of Artemisia alba Turra through plant tissue culture techniques', Plant Cell, Tissue and Organ Culture (PCTOC), 157(29). doi: 10.1007/s11240-024-02756-y.
• Vahekeni, N. et al. (2024) 'Antiprotozoal activity of plants used in the management of sleeping sickness in Angola and bioactivity-guided fractionation of Brasenia schreberi J.F.Gmel and Nymphaea lotus L. active against T. b. rhodesiense', Molecules, 29(7), p. 1611. doi: 10.3390/molecules29071611.
• Meier, N. et al. (2024) 'Photostability of sennosides and their aglycones in solution', Phytochemical Analysis, 35(3), pp. 521–529. doi: 10.1002/pca.3308.
• Silva Oliveira, A. G. et al. (2023) 'Tapirira guianensis is selectively cytotoxic, induces apoptosis to the glioblastoma and decreases tumor growth and angiogenesis in vivo', Planta Medica, 90(1), pp. 13–24. doi: 10.1055/a-2181-2569.
• Trifan, A. et al. (2023) 'Influence of pyrrolizidine alkaloids depletion upon the biological activity of Symphytum officinale L. extracts', Journal of Ethnopharmacology, 303(116010). doi: 10.1016/j.jep.2022.116010.
• Lardos, A. et al. (2022) 'Computational literature-based discovery for natural products research : current state and future prospects', Frontiers in Bioinformatics, 2(827207). doi: 10.3389/fbinf.2022.827207.
• Trifan, A. et al. (2021) 'Influence of the post-harvest storage time on the multi-biological potential, phenolic and pyrrolizidine alkaloid content of comfrey (Symphytum officinale L.) roots collected from different European regions', Plants, 10(9), p. 1825. doi: 10.3390/plants10091825.
• Pihan, L.-A.-M. et al. (2021) 'HPTLC fingerprint authentication of selected Sideritis spp. using a pharmacognostic approach', Planta Medica, 87(14), pp. 1152–1166. doi: 10.1055/a-1647-2930.
• Trifan, A. et al. (2021) 'LC-HRMS/MS phytochemical profiling of Symphytum officinale L. and Anchusa ochroleuca M. Bieb. (Boraginaceae) : unveiling their multi-biological potential via an integrated approach', Journal of Pharmaceutical and Biomedical Analysis, 204, p. 114283. doi: 10.1016/j.jpba.2021.114283.
• Trifan, A. et al. (2020) 'Globoidnan A, rabdosiin and globoidnan B as new phenolic markers in European‐sourced comfrey (Symphytum officinale L.) root samples', Phytochemical Analysis, 32(4), pp. 482–494. doi: 10.1002/pca.2996.
• Iliescu, I. A. et al. (2020) 'Verbascum nigrum : cytotoxicity evaluation in A431 epidermoid carcinoma cells and untargeted LC‐HR‐MS/MS metabolite profiling', Chemistry & Biodiversity, 17(12), p. e2000644. doi: 10.1002/cbdv.202000644.
• Trifan, A. et al. (2020) 'Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils', Journal of Ethnopharmacology, 262(113169). doi: 10.1016/j.jep.2020.113169.
• Ribeiro Neto, J. A. et al. (2020) 'Using the plants of Brazilian Cerrado for wound healing : from traditional use to scientific approach', Journal of Ethnopharmacology, 260(112547). doi: 10.1016/j.jep.2020.112547.
• Stuby, J. et al. (2019) 'Appetite-suppressing and satiety-increasing bioactive phytochemicals : a systematic review', Nutrients, 11(9), p. 2238. doi: 10.3390/nu11092238.
• Trifan, A. et al. (2019) 'Unravelling the potential of seaweeds from the Black Sea coast of Romania as bioactive compounds sources : part I: Cystoseira barbata (Stackhouse) C. Agardh', Food and Chemical Toxicology, 134(110820). doi: 10.1016/j.fct.2019.110820.
• Navikaite-Snipaitiene, V. et al. (2018) 'Development of antioxidant food packaging materials containing eugenol for extending display life of fresh beef', Meat Science, 145, pp. 9–15. doi: 10.1016/j.meatsci.2018.05.015.
• Trendafilova, A. et al. (2018) 'Phenolic profile of Artemisia alba Turra', Chemistry & Biodiversity, 15(7). doi: 10.1002/cbdv.201800109.
• Bucar, F. and Wolfram, E. (2017) 'Bioassay-coupled chromatography : challenges and applications in natural product research', Phytochemical Analysis, 28(2), p. 73. doi: 10.1002/pca.2675.
• Peter, S. et al. (2017) 'High-Performance thin-layer chromatographic fingerprint method for the detection of sennosides in Cassia senna L. and Cassia angustifolia Vahl', Journal of Planar Chromatography - Modern TLC, 30(4), pp. 238–244. doi: 10.1556/1006.2017.30.4.1.
• Trifan, A. et al. (2017) 'Is comfrey root more than toxic pyrrolizidine alkaloids? Salvianolic acids among antioxidant polyphenols in comfrey (Symphytum officinale L.) roots', Food and Chemical Toxicology, 112, pp. 178–187. doi: 10.1016/j.fct.2017.12.051.
• Meier, N. et al. (2017) 'In-Silico UHPLC method optimization for aglycones in the herbal laxatives aloe barbadensis Mill., Cassia angustifolia Vahl Pods, Rhamnus frangula L. Bark, Rhamnus purshianus DC. Bark, and Rheum palmatum L. Roots', Molecules, 22(11). doi: 10.3390/molecules22111838.
• Wolfram, E. and Bräm, S. (2017) 'Recent advances in effect-directed enzyme assays based on thin-layer chromatography', Phytochemical Analysis, 28(2), pp. 74–86. doi: 10.1002/pca.2669.
• Peter, S. et al. (2016) 'Optimization of LC separation of anthraquinones in Sennae fructus and Sennae folium supported by an in-silico tool', Planta Medica, 81(S 01). doi: 10.1055/s-0036-1597006.
• Steiner, M. et al. (2016) 'Modernisierung der Monografien für Thymianfluidextrakt und Thymiansirup der Schweizer Pharmakopöe', Zeitschrift für Phytotherapie, 37(P24). doi: 10.1055/s-0036-1584487.
• Braun, K. et al. (2016) 'Untersuchungen zum Extraktionsverhalten der Anthranoide in Sennae folium/fructus unter Berücksichtigung der Aglyka', Zeitschrift für Phytotherapie, 37(V30). doi: 10.1055/s-0036-1584455.
• Pedrussio, S., Kroslakova, I. and Wolfram, E. (2016) 'Direct coupling of HPTLC with MALDI-TOF MS for qualitative detection of flavonoids on phytochemical fingerprints', Phytochemical Analysis, 27(3-4), pp. 222–228. doi: 10.1002/pca.2621.
• Danova, K. et al. (2016) 'Biotechnological yield of phytopharmaceuticals of valuable Balkan medicinal plants', Planta Medica, 82(S 01). doi: 10.1055/s-0036-1596790.
• Wolfram, E. et al. (2016) 'HPTLC and HPTLC-coupled bioassays for bioprocess control of medicinal plant in vitro cultures from the Balkan region', Planta Medica, 82(S 01). doi: 10.1055/s-0036-1596387.
• Todorova, M. et al. (2015) 'Highly oxygenated sesquiterpenes in Artemisia alba Turra', Phytochemistry, 110, pp. 140–149. doi: 10.1016/j.phytochem.2014.12.008.
• Meier, N. et al. (2015) 'Content of free anthraquinone aglyca in anthranoid-containing herbal drugs/laxatives of the European Pharmacopoeia (Ph. Eur.)', Planta Medica, 81(PW_196). doi: 10.1055/s-0035-1565820.
• Iliescu, I. A. et al. (2015) 'Phytochemical fingerprints combined with bioautographic enzyme inhibition and antitumor activity of Verbascum (Scrophulariaceae) and Ajuga (Lamiaceae) plant extractives on skin squamous cell carcinoma', Planta Medica, 81(PW_208). doi: 10.1055/s-0035-1565832.
• González, B. et al. (2015) 'Polyphenol, anthocyanin and antioxidant content in different parts of maqui fruits (Aristotelia chilensis) during ripening and conservation treatments after harvest', Industrial Crops and Products, 76, pp. 158–165. doi: 10.1016/j.indcrop.2015.06.038.
• Wolfram, E. et al. (2015) 'Enzymatic bioautography on HPTLC : combined phytochemical and activity screening tool for quality assessment and in vitro cultivation bioprocess control for selected medicinal plants from the Balkan region', Planta Medica, 81(SL4B_05). doi: 10.1055/s-0035-1565330.
• Könye, R. et al. (2015) 'Optimization of an UHPLC method for flavonoids from Hypericum species', Planta Medica, 81(PW_194). doi: 10.1055/s-0035-1565818.
• Danova, K. et al. (2015) 'Relations between polyphenolics production and enzymatic antioxidant defense in Pulsatilla montana ssp. balcana in vitro', Planta Medica, 81(PW_04). doi: 10.1055/s-0035-1565628.
• Koleva, P. et al. (2015) 'In vitro culture development and polyphenolics production of Artemisia alba Turra', Journal of Biosciences and Biotechnology, pp. 131–136. doi: 10.21256/zhaw-4128.
• Rosenthal, M. I. et al. (2014) 'Validated method for the analysis of frangulins A and B and glucofrangulins A and B using HPLC and UHPLC', Journal of Natural Products, 77(3), pp. 489–496. doi: 10.1021/np400736s.
• Ştefanache, C. P. et al. (2014) 'In situ and in vitro comparative study on the bioproductivity of Arnicae folium et caulis from the northern area of the Romanian eastern carpathians', Journal of Plant Development, 21, pp. 3–13. doi: 10.21256/zhaw-1695.
• Treneva, G. et al. (2014) 'Effect of plant growth regulators on growth patterns and enzymatic antioxidant activities in Hypericum calycinum shoot cultures', Bulgarian Journal of Agricultural Science, 20(Supplement 1), pp. 46–50. doi: 10.21256/zhaw-4132.
• Wolfram, E. et al. (2014) 'Bioautographic screening of Xanthine Oxidase inhibition and antioxidant activities for bioprocess control of in vitro cultivated medicinal and aromatic plants of the Balkan region', Planta Medica, 80(16/P2B62). doi: 10.1055/s-0034-1394939.
• Rosenthal, M. I., Wolfram, E. and Meier, B. (2013) 'New UHPLC – method to determine Aloin A and B in Aloe capensis', Planta Medica, 79(PK34). doi: 10.1055/s-0033-1352294.
• Rosenthal, M. I., Wolfram, E. and Meier, B. (2013) 'New HPLC-method to determine Frangulin A and B as well as Glucofrangulin A and B in Frangulae cortex', Planta Medica, 79(13/PK33). doi: 10.1055/s-0033-1352293.
• Stefanache, C. P. et al. (2013) 'Arnica montana L. cell suspension culture as a biotechnological approach for the production of bioactive secondary metabolites', Planta Medica, 79(13). doi: 10.1055/s-0033-1352447.
• Todorova, M. et al. (2013) 'Highly oxygenated sesquiterpenes from the aerial parts of Artemisia alba Turra', Planta Medica, 79(13/PI107). doi: 10.1055/s-0033-1352196.
• Danova, K. et al. (2013) 'Optimization of in vitro culture system for biomass and polyphenolics production in Inula britannica and Sideritis scardica Sofia 2 cultivar', Planta Medica, 79(13/PN21). doi: 10.1055/s-0033-1352365.
• Wolfram, E. et al. (2013) 'Phytochemical assessment of the effect of stimulated in vitro multiplication on the metabolic profile of in vitro cultured Hypericum richeri and Artemisia alba', Planta Medica, 79(13/PN119). doi: 10.1055/s-0033-1352461.
• Markovska, Y. et al. (2013) 'Enzymatic activity and physiological status affect essential oils and polyphenolics production in Artemisia alba tissue cultures', Planta Medica, 79(13/PN20). doi: 10.1055/s-0033-1352364.
• Reich, E. et al. (2007) 'HPTLC methods for identification of green tea and green tea extract', Journal of Liquid Chromatography & Related Technologies, 29(14), pp. 2141–2151. doi: 10.1080/15512160600760293.
• Haberland, J. et al. (2002) 'Diastereoselective synthesis of optically active (2R,5R)-hexanediol', Applied Microbiology and Biotechnology, 58(5), pp. 595–599. doi: 10.1007/s00253-002-0936-5.
• Garvey, E. et al. (1998) 'Coliform transport in a pristine reservoir : modeling and field studies', Water Science and Technology, 37(2), pp. 137–144. doi: 10.1016/S0273-1223(98)00048-1.

Books, peer-reviewed

Wolfram, E. P. (2002) Parallele Substratkontrolle in Bioreaktoren mit geringem Volumen. Doctoral dissertation. Forschungszentrum Jülich, Zentralbibliothek.

Book chapters, peer-reviewed

Wolfram, E. and Trifan, A. (2018) 'Computational aids for assessing bioactivities', in Sarker, S. and Nahar, L. (eds) Computational phytochemistry. Amsterdam: Elsevier, pp. 277–300. doi: 10.1016/B978-0-12-812364-5.00010-9.

Written conference contributions, peer-reviewed

• Sellathurai, V. et al. (2025) 'In vitro studies on the wound healing potential of dermatological plants from the Receptarium of Burkhard III von Hallwyl, a recipe text from 16th century Switzerland', in 73rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA), Naples, Italy, 31 August - 3 September 2025.
• Wolfram, E. and Hölzel, L. (2023) 'Swiss herbs in climate change : HPTLC fingerprints of thyme, sage and horehound from Zermatt and a controlled green house', in Planta Medica. Thieme, p. 1403. doi: 10.1055/s-0043-1774204.
• Wolfram, E. et al. (2023) 'Planar umuC assay : a novel effect-directed analysis kit for screening of genotoxic potential of plant extracts', in Planta Medica. Thieme, pp. 1396–1397. doi: 10.1055/s-0043-1774184.
• Stehlin, J. et al. (2023) 'Immunomodulatory studies on selected plants from the Hallwyl 'Kräuterbuch' : an influential recipe text from 16th century Switzerland', in Planta Medica. Thieme, pp. 1392–1393. doi: 10.1055/s-0043-1774171.
• Vahekeni, N. et al. (2023) 'Historical herbal texts from Switzerland : a potential source for plant- derived natural products against SARS-CoV-2', in Planta Medica. Thieme, p. 1422. doi: 10.1055/s-0043-1774265.
• Pihan, L.-A.-M. et al. (2019) 'Antiviral screening and bioautographic assessment of radical scavenging, estrogenic and AchE-inhibitoric activity of Sideritis species', in 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP, Innsbruck, Austria, 1-5 September 2019.
• Peter, S. et al. (2019) 'HPTLC identity testing for Rhamni purshianae cortex and isolation of Cascarosides B, C and D as reference compounds', in 67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in cooperation with the French Society of Pharmacognosy AFERP, Innsbruck, Austria, 1-5 September 2019.
• Merki, R. et al. (2019) 'Screening for active substances with TLC bioautography : challenges with artefacts', in 12th BioDetectors Conference 2019 : Abstract book. Vienna: BioDetection Systems.
• Trifan, A. et al. (2019) 'Cystoseira barbata C. Agardh from Romanian Black Sea coast : phlorotannins profile, in vitro antioxidant and antiproliferative potential', in Planta Medica. Stuttgart: Thieme, pp. 1524–1525. doi: 10.1055/s-0039-3400023.
• Wolfram, E. et al. (2017) 'Development of an HPTLC identification method in new Ph Eur monograph for Rubi ideae folium (raspberry leaves)', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608528.
• Wolfram, E. (2017) 'Contributions to regulation of herbal medicinal products from scientific institutions', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608025.
• Danova, K. et al. (2017) 'Biotechnological development and biological activity of Balkan endemic Sideritis scardica Griesb.', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608366.
• Bräm, S. and Wolfram, E. (2017) 'Evaluation of estrogenic activity of Trifolium pratense L. and Cimicifuga racemosa L. plant extracts and formulations using the planar-YES assay', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608486.
• Meier, N. et al. (2017) 'Photostability of sennosides in solution and their degradation products 1', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608273.
• Meier, N. et al. (2017) 'HPTLC fingerprint method for the detection of sennosides in Senna dry extracts', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608524.
• Schenk, A. et al. (2017) 'Combined determination of sennosides, monomeric hydroxyanthacene glycosides and anthraquinone aglycones by UHPLC-DAD', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608215.
• Wolfram, E. et al. (2017) 'Effect of growth regulators and photoperiod on endogenous phytohormonal levels and polyphenolic production in Artemisa alba cell aggregate cultures', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608367.

Other publications

• Krenn, L. et al. (2025) '35 years of the European Scientific Cooperative on Phytotherapy : successful European integration for herbal medicine', HerbalGram, (142), pp. 29–30. Available at: https://www.herbalgram.org/resources/herbalgram/issues/142/table-of-contents/hg142-orgnews-escop/.
• Vahekeni, N. et al. (2017) 'Use of herbal medicine in the management of trypanosomiasis in Angola', in Planta Medica International Open. Thieme. doi: 10.1055/s-0037-1608565.
• Yildirim, S., Wolfram, E. and Rüegg, N. (2016) 'Polysaccharide and essential oils coatings for active food packaging materials', in Baltic polymer symposium 2016: Klaipėda, September 21-24, 2016 : programme and abstracts. Kaunas: Kaunas University of Technology, p. 88.
• Wolfram, E. (2015) 'Der Medizinnobelpreis 2015 – Auswirkungen auf die Phytopharmazie?', Schweizerische Zeitschrift für Ganzheitsmedizin, 27(6), pp. 320–321. doi: 10.1159/000441870.
• Stefanache, C. P. et al. (2015) 'Phytochemical composition of Arnicae flos from wild populations in the northern area of the Romanian eastern carpathians', Revista de Chimie, 66(5), pp. 784–787.
• Wolfram, E., Peter, S. and Meier, B. (2014) 'Phytochemical analysis using HPTLC and modern LC for application in quality control and medicinal plants research : trends and tools', in Phytochemistry in Medicine and Pharmacognosy, Piatra-Neamt, 27-30 April 2014.
• Rosenthal, M. I., Wolfram, E. and Meier, B. (2014) 'An HPLC method to determine sennoside A and sennoside B in Sennae fructus and Sennae folium', Pharmeuropa Bio & Scientific Notes, pp. 92–102.
• Wolfram, E., Aus dem Siepen, B. and Lindner, M. (2013) 'Die Mangostan Frucht und ihre wirksamen Xanthone : eine aktuelle Literaturübersicht und ein Ausblick für den Einsatz in der Kosmetik', in Kosmetikjahrbuch 2014 : FIT – Formulierungen, Inhaltsstoffe, Technologie. Augsburg: SOFW.
• Treneva, G. et al. (2013) 'Effect of plant growth regulators on growth patterns and physiological status of Hypericum calycinum shoot cultures', in Book of Abstracts Vol. IV.
• Wolfram, E., Meier, B. and Danova, K. (2013) 'The Bulgarian Swiss joint research project PhytoBalk : an example of application of biotechnological methods for the conservation of valuable medicinal plants germplasm and region independent biotechnological production of plant derived pharmaceuticals', in 2nd GRF One Health Summit 2013, Davos, 17-20 November 2013.
• Wolfram, E., Spriano, D. and Meier, B. (2011) 'Extract and extract might not be the same : evaluation of the quality of plant extracts', in Kosmetikjahrbuch 2011/2012 : the green book of cosmetics. Augsburg: Verlag für Chemische Industrie.

Oral conference contributions and abstracts

• Trifan, A. et al. (2025) 'Symphytum genus : novel insights into its phytochemistry and bioactivity', in Phytochemistry Letters. Elsevier. doi: 10.1016/j.phytol.2025.103121.
• Wolfram, E. (2025) 'Phytotherapie in der Schweizer Verfassung – ein Erfolgsmodell?', in Zeitschrift für Phytotherapie. Thieme, p. S2. doi: 10.1055/s-0045-1811610.
• Wolfram, E. et al. (2025) 'Monographs and scientific evidence in the era of AI – unde venis – quo vadis ESCOP?', in Planta Medica. Thieme, pp. 215–216. doi: 10.1055/s-0045-1814906.
• Urmann, C. et al. (2025) 'Targeting the hidden potential : bioactivity-guided metabolomics for antiviral compound discovery in plants', in Planta Medica. Thieme, p. 187. doi: 10.1055/s-0045-1814833.
• Miron, A. et al. (2019) 'Plant-derived products as antibiotic enhancers and antibiotic-resistance modifying agents', in Planta Medica. Thieme, p. 1422. doi: 10.1055/s-0039-3399721.
• Wolfram, E. et al. (2013) 'HPTLC method for phytochemical and radical scavenging profiles of Chilean 'Maqui' berries (Aristotelia chilensis) at different ripening stages from cultivated accessions : abstract', in 61st International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, Münster, Germany, 1-5 September 2013. Thieme. doi: 10.1055/s-0033-1352304.

Patents and patent applications

• Wolfram, Evelyn; Franz, Andreas; Weuster-Botz, Dirk; Wandrey, Christian; Arnold, Matthias,

  2001.

  Verfahren und Vorrichtung für die Bestimmung von Substrat- und Produktkonzentrationen in einem Medium.

  Patent number DE10024992 A1

  (2001-12-06)

  .

  Available from: https://worldwide.espacenet.com/patent/search?q=pn%3DDE10024992A1

go back