MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches

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Projektleiter/in : Dr. Joël Pothier
Projektteam : Dr. Fabio Rezzonico
Projektstatus : laufend
Drittmittelgeber : Interne Förderung
Projektpartner : Mabritec AG
Kontaktperson : Joël Pothier

Beschreibung

In collaboration with Mabritec AG, we have developed a database of an unprecedented nature, the PAPMID (putative assigned protein masses for identification database). Based on over 12,000 sequences of bacterial genomes, it contains the molecular masses of the ribosomal proteins of these microorganisms and this allows for the instant identification and subtyping of bacteria. In combination with the spectra obtained by MALDI-TOF MS, this original database is an important aid in the interpretation of phylogenetic relationships between the investigated bacteria.

Not restricted to any one type of mass spectrometer, this database will soon be available to customers via the Internet.

Publikationen

Rothen, Julian; Sapugahawatte, Dulmini Nanayakkara; Li, Carmen; Lo, Norman; Vogel, Guido; Foucault, Frédéric; Pflüger, Valentin; Pothier, Joël; Blom, Jochen; Daubenberger, Claudia; Ip, Margaret,

2020.

A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS.

Scientific Reports.

10(8788).

Verfügbar unter: https://doi.org/10.1038/s41598-020-65707-5
Fossou, Romain K.; Pothier, Joël F.; Zézé, Adolphe; Perret, Xavier,

2020.

Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire.

International Journal of Systematic and Evolutionary Microbiology.

70(2), S. 1421-1430.

Verfügbar unter: https://doi.org/10.1099/ijsem.0.003931
Rothen, Julian; Pothier, Joël F.; Foucault, Frédéric; Blom, Jochen; Nanayakkara, Dulmini; Li, Carmen; Ip, Margaret; Tanner, Marcel; Vogel, Guido; Pflüger, Valentin; Daubenberger, Claudia A.,

2019.

Subspecies typing of Streptococcus agalactiae based on ribosomal subunit protein mass variation by MALDI-TOF MS.

Frontiers in Microbiology.

10(471).

Verfügbar unter: https://doi.org/10.3389/fmicb.2019.00471
Normand, Philippe; Nouioui, Imen; Pujic, Petar; Fournier, Pascale; Dubost, Audrey; Schwob, Guillaume; Klenk, Hans-Peter; Nguyen, Agnès; Abrouk, Danis; Herrera-Belaroussi, Aude; Pothier, Joël F.; Pflüger, Valentin; Fernandez, Maria P.,

2018.

Frankia canadensis sp. nov., isolated from root nodules of Alnus incana subspecies rugosa.

International Journal of Systematic and Evolutionary Microbiology.

68, S. 3001-3011.

Verfügbar unter: https://doi.org/10.1099/ijsem.0.002939
Rothen, Julian; Schindler, Tobias; Pothier, Joël F.; Younan, Mario; Certa, Ulrich; Daubenberger, Claudia; Pflüger, Valentin; Jores, Joerg,

2017.

Draft genome sequences of seven Streptococcus agalactiae strains isolated from Camelus dromedarius at the horn of Africa.

Genome Announcements.

5(28).

Verfügbar unter: https://doi.org/10.1128/genomeA.00525-17
Ziegler, Dominik; Pothier, Joël F.; Ardley, Julie; Kouakou Fossou, Romain; Pflüger, Valentin; de Meyer, Sofie; Vogel, Guido; Tonolla, Mauro; Howieson, John; Reeve, Wayne; Perret, Xavier,

2015.

Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS.

Applied Microbiology and Biotechnology.

99(13), S. 5547-5562.

Verfügbar unter: https://doi.org/10.1007/s00253-015-6515-3
van der Wolf, Jan M.; Nijhuis, Els H.; Kowalewska, Malgorzata J.; Saddler, Gerry S.; Parkinson, Neil; Elphinstone, John G.; Pritchard, Leighton; Toth, Ian K.; Lojkowska, Ewa; Potrykus, Marta; Waleron, Malgorzata; de Vos, Paul; Cleenwerck, Ilse; Pirhonen, Minna; Garlant, Linda; Hélias, Valérie; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Tsror, Leah; Manulis, Shula,

2014.

Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum).

International Journal of Systematic and Evolutionary Microbiology.

64, S. 768-774.

Verfügbar unter: https://doi.org/10.1099/ijs.0.052944-0

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