Spatio-temporal transcriptional effects of compatible pathogen attack in tomato
Numerous studies have demonstrated that plant species react at the transcriptional level to an attack by a compatible pathogen. As in incompatible reactions, the transcriptional changes are not forcefully restricted to the infection site but may extend to the surrounding tissue as well. By means of quantitative real time PCR we studied the spatial regulation of genes within tomato leaves (Solanum lycopersicon) challenged with the late blight pathogen Phytophthora infestans and the gray mold Botrytis cinerea at 24 hours post infection. In parallel the temporal regulation of tomato genes was observed in three-hour intervals during the first 24 hours of disease.