CANCELLED: Workshop: 3D YOU CAN SEE – PLANAR 3D SPHEROID ARRAYS FOR HISTOLOGY
19 February 2019
In this collaborative workshop in London with TEDD Partner Kugelmeiers we will show how to to do histological assessment of 3D spheroids like never before.
WORKSHOP HAS BEEN CANCELLED BY THE ORGANIZERS
February 19th, 2019 | London, United Kingdom
Certain standard procedures are indispensable for life sciences. Histology is one of them. Now, with 3D spheroids, a model to closely mimic human physiology for a vast array of applications, is finally broadly available. But for 3D spheroids, histological assessment so far has been done as irregular bulk without a clear optical plane.
Thanks to the totally regular array spheroid building in the Sphericalplate 5D, we will show how to catch spheroids with polymerizing gel and processing histology to a planar array of 750 spheroids. This perfectly fits a microscope slide and allows the user to do histological assessment of 3D spheroids like never before.
Benefits of attending
- If you do histology on 3D spheroids, this workshop is for you. It offers you a new dimension of scalability and analysis flow.
- Various research areas like cancer screening and stem cell research need an individual understanding of spheroid biology – cut in one plane x 750 samples gives you unparalleled data accuracy.
- Statistical power will be much higher, as you can rely on more, regular data points.
- Various showcases like hypoxia effects on spheroid biology or cell population heterogeneity can be studied more accurately when spheroids are organised in one plane.
Patrick Kugelmeier, Founder, Director of Science, Kugelmeiers AG
Michael Raghunath, Professor for Biochemistry, Protein Technology and Tissue Engineering, Zurich University of Applied Sciences
Patrick Kugelmeier studied medicine and did his medical thesis in islet transplantation for the treatment of Diabetes. The joy of research led to an MD-PhD program for the early differentiation of stem cells. Cell culture technologies at that time didn't allow for good data reproducibility, because stem cells were cultured in 2D. Fueled by the clinical need of small, size-controlled cell clusters to improve islet transplantation and the insights from the sensitivity of stem cells, he began to develop what was later to become the Sphericalplate 5D. After the MD-PhD program, he did his clinical training in visceral and transplant surgery followed by trauma surgery. Besides the clinical work, the Sphericalplate 5D was further developed. Finally, it became clear that this technology opens a new dimension in cell transplantation and regenerative medicine and Dr. Kugelmeier decided to quit surgery to fully live this vision within a startup-company, the Kugelmeiers AG.
Michael Raghunath is a clinical scientist and internationally renowned for work in matrix biology, wound healing, and implementing the biophysical principle of macromolecular crowding in tissue engineering. After completing medical studies in Mainz, Germany, he received training in immunology at the University of Heidelberg, Germany, and as a postdoc in matrix biology and wound healing at the University of Zurich, Switzerland. In 1997, he received his habilitation in Physiological Chemistry and Pathobiochemistry at the University of Münster, Germany, and did a clinical residency in dermatology there. After a stint in industry as R&D director, he became an Associate Professor of Biomedical Engineering and Biochemistry in 2003 at the National University of Singapore. In 2016, he joined ZHAW as Professor and Head, Center of Cell Biology & Tissue Engineering. He currently holds 123 research publications (h-index: 42; 4813 cites) and several review articles and book chapters. He is member of the EU Council for TERMIS and in the editorial board of Current Opinion in Biomedical Engineering, Journal of Tissue Engineering and Regenerative Medicine, and International Journal of Bioprinting. As Director of the Competence Center for Tissue Engineering (TEDD) he has a strong interest in 3D tissue models to replace animal experimentation.
Start date: 19 February 2019
End date: 19 February 2019