Dr. Valentin Jossen

Position at the ZHAW

- Research Associate
- Deputy head of the Cell Culture Technology Group

www.zhaw.ch/icbt/bioverfahrenstechnik

Expertise and research interests

- Cell Culture Technique (mammalian cells, human stem cells)
- Computational Fluid Dynamics (CFD)
- Biochemical Engineering characterization and development of new bioreactor systems

Educational background

- Dr.-Ing. (Technical University of Berlin)
- Certificate of Advanced Studies in didactics (Zurich University of Education)
- M.Sc. Pharmaceutical Biotechnology (Zurich University of Applied Sciences)
- B.Sc. Biotechnology (Zurich University of Applied Sciences)

Membership of networks

• DECHEMA

• Muoio, Francesco; Panella, Stefano; Lindner, Matias; Jossen, Valentin; Harder, Yves; Moccetti, Tiziano; Eibl-Schindler, Regine; Müller, Michele; Tallone, Tiziano,

  2021.

  Development of a biodegradable microcarrier for the cultivation of human adipose stem cells (hASCs) with a defined xeno- and serum-free medium.

  Applied Sciences.

  11(3), pp. 925.

  Available from: https://doi.org/10.3390/app11030925

• Panella, Stefano; Muoio, Francesco; Jossen, Valentin; Harder, Yves; Eibl-Schindler, Regine; Tallone, Tiziano,

  2021.

  Chemically defined xeno- and serum-free cell culture medium to grow human adipose stem cells.

  Cells.

  10(2), pp. 466.

  Available from: https://doi.org/10.3390/cells10020466

• Muoio, Francesco; Panella, Stefano; Jossen, Valentin; Lindner, Matias; Harder, Yves; Müller, Michele; Eibl, Regine; Tallone, Tiziano,

  2021.

  Human adipose stem cells (hASCs) grown on biodegradable microcarriers in serum- and xeno-free medium preserve their undifferentiated status.

  Journal of Functional Biomaterials.

  12(2), pp. 25.

  Available from: https://doi.org/10.3390/jfb12020025

• Seidel, Stefan; Maschke, Rüdiger; Werner, Sören; Jossen, Valentin; Eibl, Dieter,

  2020.

  Oxygen mass transfer in biopharmaceutical processes : numerical and experimental approaches .

  Chemie Ingenieur Technik.

  93(1-2).

  Available from: https://doi.org/10.1002/cite.202000179

• Jossen, Valentin; Muoio, Francesco; Panella, Stefano; Harder, Yves; Tallone, Tiziano; Eibl-Schindler, Regine,

  2020.

  An approach towards a GMP compliant in-vitro expansion of human adipose stem cells for autologous therapies.

  Bioengineering.

  7(3), pp. 77.

  Available from: https://doi.org/10.3390/bioengineering7030077

• Jossen, Valentin; Eibl-Schindler, Regine; Kraume, Matthias; Eibl, Dieter,

  2018.

  Growth behavior of human adipose tissue-derived stromal/stem cells at small scale : numerical and experimental investigations.

  Bioengineering.

  5(4), pp. 106.

  Available from: https://doi.org/10.3390/bioengineering5040106

• Eibl-Schindler, Regine; Jossen, Valentin; Eibl, Dieter,

  2018.

  Einweg-Bioreaktoren : therapeutische Antikörper.

  Chemie in unserer Zeit.

  52(4), pp. 230-237.

  Available from: https://doi.org/10.1002/ciuz.201800803

• Jossen, Valentin; van den Bos, Christian; Eibl-Schindler, Regine; Eibl, Dieter,

  2018.

  Manufacturing human mesenchymal stem cells at clinical scale : process and regulatory challenges.

  Applied Microbiology and Biotechnology.

  102(9), pp. 3981-3994.

  Available from: https://doi.org/10.1007/s00253-018-8912-x

• Kaiser, Stephan C.; Werner, Sören; Jossen, Valentin; Kraume, Matthias; Eibl, Dieter,

  2017.

  Development of a method for reliable power input measurements in conventional and single-use stirred bioreactors at laboratory scale.

  Engineering in Life Sciences.

  17(5), pp. 500-511.

  Available from: https://doi.org/10.1002/elsc.201600096

• Demuth, Caspar; Varonier, Joel; Jossen, Valentin; Eibl-Schindler, Regine; Eibl, Dieter,

  2016.

  Novel probes for pH and dissolved oxygen measurements in cultivations from millilitre to benchtop scale.

  Applied Microbiology and Biotechnology.

  100(9), pp. 3853-3863.

  Available from: https://doi.org/10.1007/s00253-016-7412-0

• Jossen, Valentin; Schirmer, Cedric; Mostafa Sindi, Dolman; Eibl-Schindler, Regine; Kraume, Matthias; Pörtner, Ralf; Eibl, Dieter,

  2016.

  Theoretical and practical issues that are relevant when scaling up hMSC microcarrier production processes.

  Stem Cells International.

  2016(4760414).

  Available from: https://doi.org/10.1155/2016/4760414

• Schirmaier, Carmen; Jossen, Valentin; Kaiser, Stephan C.; Jüngerkeks, Frank; Silke, Brill; Safavi-Nab, Agnieszka; Siehoff, Ann; van den Bos, Christian; Eibl, Dieter; Eibl-Schindler, Regine,

  2014.

  Scale-up of adipose tissue-derived mesenchymal stem cell production in stirred single-use bioreactors under low-serum conditions.

  Engineering in Life Sciences.

  14(3), pp. 292-303.

  Available from: https://doi.org/10.1002/elsc.201300134

• Jossen, Valentin; Kaiser, Stephan C.; Schirmaier, Carmen; Hermann, Jacqueline; Tappe, Alexander; Eibl, Dieter; Siehoff, Ann; van den Bos, Christian; Eibl-Schindler, Regine,

  2014.

  Modification and qualification of a stirred single-use bioreactor for the improved expansion of human mesenchymal stem cells at benchtop scale.

  Pharmaceutical Bioprocessing.

  2(4), pp. 311-322.

• Kaiser, Stephan; Jossen, Valentin; Schirmaier, Carmen; Eibl, Dieter; Brill, Silke; van den Bos, Christian; Eibl-Schindler, Regine,

  2013.

  Fluid flow and cell proliferation of mesenchymal adipose-derived stem cells in small-scale, stirred, single-use bioreactors.

  Chemie Ingenieur Technik.

  85(1-2), pp. 95-102.

  Available from: https://doi.org/10.1002/cite.201200180

• Jossen, Valentin; Eibl, Dieter; Eibl-Schindler, Regine,

  2020.

  Numerical methods for the design and description of in vitro expansion processes of human mesenchymal stem cells

  .

  In:

  Advances in Biochemical Engineering/Biotechnology.

  Berlin:

  Springer.

  Available from: https://doi.org/10.1007/10_2020_147

• Jossen, Valentin; Eibl-Schindler, Regine; Pörtner, Ralf; Kraume, Matthias; Eibl, Dieter,

  2017.

  Stirred bioreactors : current state and developments, with special emphasis on biopharmaceutical production processes

  .

  In:

  Current Developments in Biotechnology and Bioengineering.

  Elsevier.

  pp. 179-215.

  Available from: https://doi.org/10.1016/B978-0-444-63663-8.00007-0

• Maschke, Rüdiger; Werner, Sören; Stalder, Mischa; Jossen, Valentin; Eibl, Dieter,

  2016.

  Nutzung von Sensor Spots zur Bestimmung der Gelöstsauerstoffkonzentration in Single-use Systemen im Labormassstab [poster].

  In:

  Institut für Bioprozess- und Analysenmesstechnik, ed.,

  Tagungsband.

  18. Heiligenstädter Kolloquium "Technische Systeme für die Lebenswissenschaft", Heilbad Heiligenstadt, 19.-21. September 2016.

  Winterthur:

  ZHAW Zürcher Hochschule für Angewandte Wissenschaften.

  Available from: https://doi.org/10.21256/zhaw-1152

• Jossen, Valentin; Eberle, Sebastian; Muoio, Francesco; Panella, Stefano; Tallone, Tiziano; Moccetti, Tiziano; Lindner, Matias; Müller, Michele; Eibl, Dieter; Eibl-Schindler, Regine; et al.,

  2019.

  Serum-free hMSC expansion in stirred single-use cultivation systems [poster].

  In:

  BioTech 2019, Wädenswil, Switzerland, 2-4 July 2019.

• van den Bos, Christian; Jossen, Valentin; Eibl, Dieter; Eibl-Schindler, Regine,

  2019.

  Advanced therapy medicinal products (ATMPs) & GMP : 10 years after.

  Die Pharmazeutische Industrie : pharmind.

  81(4), pp. 508-513.

• Jossen, Valentin; Eibl-Schindler, Regine; Eibl, Dieter,

  2019.

  Single‐use bioreactors : an overview

  .

  In:

  Single‐Use Technology in Biopharmaceutical Manufacture.

  Wiley.

  pp. 37-52.

  Available from: https://doi.org/10.1002/9781119477891.ch4

• Jossen, Valentin; Eibl-Schindler, Regine; Kraume, Matthias; Eibl, Dieter,

  2018.

  Growth behavior of human adipose tissue-derived stromal/stem cells in single-use spinner flasks : numerical and experimental investigations [poster].

  In:

  ECI: Single Use Technologies III: Scientific and Technological Advancements, Utah, USA, 23-26 September 2018.

• Jossen, Valentin; Schirmer, Cedric; Eibl-Schindler, Regine; Kraume, Matthias; Pörtner, Ralf; Eibl, Dieter,

  2016.

  Expansion of human adipose tissue-derived stromal/stem cells in a wave-mixed single-use bioreactor : a proof-of-concept [poster].

  In:

  BioTech 2016, Wädenswil, Switzerland, 5-8 September 2016.

• Jossen, Valentin; Eibl-Schindler, Regine; Kraume, Matthias; Pörtner, Ralf; Eibl, Dieter,

  2016.

  Impact of shear stress on cell growth and microcarrier-cell-agglomerate formation in microcarrier-based cultivations of adipose tissue-derived stromal/stem cells [poster].

  In:

  BioTech 2016, Wädenswil, Switzerland, 5-8 September 2016.

• Jossen, Valentin; Schirmer, Cedric; Eibl-Schindler, Regine; Kraume, Matthias; Pörtner, Ralf; Eibl, Dieter,

  2016.

  How to sucessfully scale up a microcarrier based expansion process of human mesenchymal stem cells from adipose tissue [poster].

  In:

  4th BioProScale Symposium 2016, Berlin, Germany, 6-8 April 2016.

• Jossen, Valentin; Eibl, Dieter,

  2015.

  Fluid flow modelling through a porous scaffold for the evaluation of co-culture model of human mesenchymal stromal cells and human dermal microvascular endothelial cells for bone tissue engineering [poster].

  In:

  24th European Society for Animal Cell Techonology (ESACT) Meeting, Barcelona, Spain, 31 May - 3 June 2015.

• Jossen, Valentin; Schirmaier, Carmen; John, Gernot T.; Eibl, Dieter; Eibl-Schindler, Regine,

  2015.

  On-line-pH- und -DO-Messungen in Mikrocarrier-basierter hMSC Kultur.

  BIOspektrum.

  21(4), pp. 422-424.

  Available from: https://doi.org/10.1007/s12268-015-0597-4

• Jossen, Valentin; Pörtner, Ralf; Kaiser, Stephan C.; Kraume, Matthias; Eibl, Dieter; Eibl-Schindler, Regine,

  2014.

  Mass production of mesenchymal stem cells — impact of bioreactor design and flow conditions on proliferation and differentiation

  .

  In:

  Eberli, Daniel, ed.,

  Cells and Biomaterials in Regenerative Medicine.

  InTech.

  pp. 119-172.

  Tissue Engineering and Regenerative Medicine.

  Available from: https://doi.org/10.5772/59385

• Jossen, Valentin; Schirmaier, Carmen; John, Gernot; Siehoff, Ann; Eibl, Dieter; Eibl-Schindler, Regine,

  2014.

  Online pH and DO measurement in microcarrier-based hMSC cultivations performed in single-use spinner flask [poster].

  In:

  BioTech 2014, Wädenswil, Switzerland, 4-5 September 2014.

• Jossen, Valentin; Ott, Anita; Kaiser, Stephan; Brill, Silke; Spicker, Sonja; Safavi-nab, Agnieszka; Jüngerkes, Frank; Siehoff, Ann; Rosenbaum, Claudia; Reinisch, Barbara; van den Bos, Christian; Eibl, Dieter; Eibl-Schindler, Regine; et al.,

  2014.

  Microcarrier-based expansion of adipose tissue and bone marrow derived human mesenchymal stem cells in therapeutically relevant doses [poster].

  In:

  4th Lugano Stem Cell Meeting, Lugano, Switzerland, 23-24 June 2014.

• Jossen, Valentin; Kaiser, Stephan; Schirmaier, Carmen; van den Bos, Christian; Eibl, Dieter; Eibl-Schindler, Regine,

  2013.

  Investigations of fluif flow and microcarrier suspension for hMSC expansion in single-use stirred bioreactors [poster].

  In:

  BioTech 2013, Wädenswil, Switzerland, 3-5 June 2013.

• Jossen, Valentin; Eibl-Schindler, Regine; Eibl, Dieter,

  2018.

  Multiphase CFD simulations for supporting MC-based hMSC process development.

  In:

  5th BioProScale Symposium, Berlin, Germany, 20-22 March 2018.

• Jossen, Valentin; Schirmer, Cedric; Eibl-Schindler, Regine; Kraume, Matthias; Pörtner, Ralf,

  2017.

  Standardized expansion of human adipose tissue-derived stromal/stem cells (hASCs) in wave-mixed single-use bioreactors with one-dimensional motion.

  In:

  ECI: Single Use Technologies II: Bridging Polymer Science to Bioprocess Applications, Tomar, Portugal, 7–10 May 2017.

  Available from: https://pdfs.semanticscholar.org/9a61/98bcd2e950104f1457e8f0e7c7380323ebf5.pdf

• Jossen, Valentin; Eibl-Schindler, Regine; Eibl, Dieter,

  2017.

  How to use Computational Fluid Dynamics in the development of cell therapeutics.

  In:

  ECI: Scale-up and Manufacturing of Cell-based Therapies V, San Diego, USA, 15-19 January 2017.

• Jossen, Valentin; Eibl, Dieter,

  2016.

  Fluid flow simulations in a porous scaffold to improve co-cultivation of human mesenchymal stem cells and human microvascular endothelial cells for bone tissue engineering.

  In:

  BMT Dreiländertagung, Basel, Switzerland, 4-6 October 2016.

• Jossen, Valentin,

  2016.

  Overview : single-use technologies in biopharmaceutical manufacturing.

  In:

  10th AchemAsia, Beijing, China, 9-12 May 2016.

• Jossen, Valentin,

  2016.

  Scale-up considerations for cell therapies.

  In:

  Informa conference "Cell therapy manufacturing & gene therapy", Brussels, Belgium, 2-3 December 2015.

• Schirmer, Cedric; Jossen, Valentin; Mostafa Sindi, Dolman; Eibl-Schindler, Regine; Böttcher, Lars; Eibl, Dieter,

  2016.

  Standardized expansion of human mesenchymal stem cells in wave-mixed single-use bioreactors.

  In:

  ILMAC, Basel, 2016.

• Jossen, Valentin,

  2015.

  Successfully employing single-use bioreactors for different expression systems.

  In:

  ECI: Single Use Technologies I: Bridging Polymer Science to Bioprocess Applications, Leesburg, USA, 18-21 October 2015.

• Jossen, Valentin; Eibl, Dieter; Eibl-Schindler, Regine,

  2015.

  How can Computational Fluid Dynamics be used in order to rapidly expand bone marrow derived hMSCs in clinically relevant numbers.

  In:

  DECHEMA Himmelfahrtstagung, Hamburg, Germany, 11-13 May 2015.

• Eibl-Schindler, Regine; Jossen, Valentin; Eibl, Dieter,

  2015.

  Mass production of hMSCs : an approach based on stirred single-use bioreactors.

  In:

  ECI: Scale-up and Manufacturing of Cell-based Therapies III, 18-22 January 2015.

• Jossen, Valentin; Kaiser, Stephan; Siehoff, Ann; van den Bos, Christian; Eibl, Dieter; Eibl-Schindler, Regine,

  2014.

  A successful approach in order to rapidly scaling-up microcarrier-based expansion of human mesenchymal stem cells for allogeneic therapies.

  In:

  3rd International Conference on Tissue Science & Regenerative Medicine, Valencia, Spain, 24-26 September 2014.

• Jossen, Valentin; Kaiser, Stephan; Eibl-Schindler, Regine; Eibl, Dieter,

  2014.

  Numerische Strömungsuntersuchungen für die Massstabsübertragung der Expansion von humanen mesenchymalen Stammzellen in gerührten Single-Use Bioreaktoren.

  In:

  17. Köthener-Rührer-Kolloquium, Köthen, Deutschland, 26. Juni 2014.

• Jossen, Valentin; Kaiser, Stephan; Ott, Anita; Siehoff, Ann; Brill, Silke; Greller, Gerhard; Tappe, Alexander; Eibl-Schindler, Regine; Eibl, Dieter; et al.,

  2014.

  Optimization of a benchtop single-use bioreactor for the expansion of adipose-tissue and bone marrow derived human mesenchymal stem cells.

  In:

  41st Annual European Society for Artifical Organs Congress, Rome, Italy, 17-20 September 2014.

• Jossen, Valentin,

  2014.

  Scale-up of hMSC cultivation processes in stirred, single-use bioreactors.

  In:

  Informa conference "Cell therapy manufacturing", Brussels, Belgium, 3-4 December 2014.

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